Dumke Roger, de la Cruz Barron Magali, Oertel Reinhard, Helm Björn, Kallies Rene, Berendonk Thomas U, Dalpke Alexander
Institute of Medical Microbiology and Virology, Technische Universität Dresden, Fetscherstrasse 74, 01307 Dresden, Germany.
Institute of Hydrobiology, Technische Universität Dresden, Helmholtzstrasse 10, 01069 Dresden, Germany.
Pathogens. 2021 Feb 12;10(2):195. doi: 10.3390/pathogens10020195.
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.
将基于废水的流行病学作为记录和管理人群中SARS-CoV-2感染过程的工具，需要了解从废水中浓缩病毒方法的效率信息。在本研究中，我们用定量的SARS-CoV-2阳性临床样本对未经处理的废水进行加标，并通过聚乙二醇沉淀和使用截留分子量为10 kDa的Vivaspin柱进行超滤来富集病毒。通过逆转录定量PCR（E基因和S基因）和数字液滴PCR对SARS-CoV-2进行检测和定量。沉淀法浓缩病毒的平均回收率在59.4%至63.7%之间，而样品超滤后的回收率为33.0%至42.6%。结果表明，两种方法的使用都能有效地、切实可行地从原废水中富集SARS-CoV-2。
