Rusiñol Marta, Martínez-Puchol Sandra, Forés Eva, Itarte Marta, Girones Rosina, Bofill-Mas Sílvia
Section of Microbiology, Virology and Biotechnology, Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona, Catalonia, Spain.
GHS, Institute of Environmental Assessment & Water Research (IDAEA), CSIC, Barcelona, Spain.
Curr Opin Environ Sci Health. 2020 Oct;17:21-28. doi: 10.1016/j.coesh.2020.08.002. Epub 2020 Aug 19.
As the novel SARS-CoV-2 was detected in faeces, environmental researchers have been using centrifugal ultrafiltration, polyethylene glycol precipitation and aluminium hydroxide flocculation to describe its presence in wastewater samples. High recoveries (up to 65%) are described with electronegative filtration when using surrogate viruses, but few literature reports recovery efficiencies using accurate quantification of enveloped viruses. Considering that every single virus will have a different behaviour during viral concentration, it is recommended to use an enveloped virus, and if possible, a betacoronaviruses as murine hepatitis virus, as a surrogate. In this review, we show new data from a newly available technology that provides a quick ultrafiltration protocol for SARS-CoV-2. Wastewater surveillance is an efficient system for the evaluation of the relative prevalence of SARS-CoV-2 infections in a community, and there is the need of using reliable concentration methods for an accurate and sensitive quantification of the virus in water.
由于在粪便中检测到新型严重急性呼吸综合征冠状病毒2（SARS-CoV-2），环境研究人员一直在使用离心超滤、聚乙二醇沉淀和氢氧化铝絮凝法来描述其在废水样本中的存在情况。使用替代病毒进行阴性电滤时回收率较高（高达65%），但很少有文献报道使用准确的包膜病毒定量法的回收效率。考虑到每种病毒在病毒浓缩过程中的行为都不同，建议使用包膜病毒，如有可能，使用β冠状病毒如鼠肝炎病毒作为替代物。在本综述中，我们展示了一项新技术的新数据，该技术为SARS-CoV-2提供了一种快速超滤方案。废水监测是评估社区中SARS-CoV-2感染相对流行程度的有效系统，需要使用可靠的浓缩方法对水中的病毒进行准确和灵敏的定量。
