Inouye M, Mio T, Sumino K
Department of Public Health, Kobe University School of Medicine, Japan.
Jpn J Med. 1988 Feb;27(1):29-33. doi: 10.2169/internalmedicine1962.27.29.
A highly sensitive and specific quantitative assay for inositol in serum has been developed. Uremic serum, to which hexahydroxybenzene as an internal standard was added, was deproteined with trichloroacetate. The supernatant aqueous phase was taken after lipids in serum were removed with chloroform, and was lyophilized. The dried powder was converted to butylboronate derivatives by reaction with 5% n-butylboronate (pyridine solution). The reaction mixture was gas chromatographed on fused silica megabore column DB-1, and assayed by electron impact mass spectrometry using selected ion monitoring. Inositol was quantified by the internal standard method. It was possible to determine concentrations as low as 0.1 microgram/ml in 0.5 ml of serum sample with a relative standard deviation of less than 5%. The serum inositol concentration from fifteen fasting patients with chronic renal failure was 39.1 +/- 18.2 micrograms/ml, significantly higher than that of control subjects (5.1 +/- 2.1 micrograms/ml). In the fifteen fasting patients, serum inositol was linearly related to blood urea nitrogen (r = 0.96).
已开发出一种用于血清中肌醇的高灵敏度和特异性定量测定方法。向尿毒症血清中加入六羟基苯作为内标，用三氯乙酸进行脱蛋白处理。用氯仿去除血清中的脂质后，取上清水相并冻干。通过与5%正丁基硼酸（吡啶溶液）反应，将干燥粉末转化为硼酸丁酯衍生物。反应混合物在熔融石英大口径柱DB-1上进行气相色谱分析，并使用选择离子监测通过电子轰击质谱法进行测定。肌醇通过内标法进行定量。在0.5 ml血清样品中能够测定低至0.1微克/毫升的浓度，相对标准偏差小于5%。15名空腹慢性肾衰竭患者的血清肌醇浓度为39.1±18.2微克/毫升，显著高于对照组（5.1±2.1微克/毫升）。在这15名空腹患者中，血清肌醇与血尿素氮呈线性相关（r = 0.96）。
