拮抗解淀粉芽孢杆菌 HX-140 的分离鉴定及其对黄瓜幼苗枯萎病的生防潜力。

Isolation and characterization of antagonistic Paenibacillus polymyxa HX-140 and its biocontrol potential against Fusarium wilt of cucumber seedlings.

机构信息

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No.348 Xianjiahu West Road, Changsha, 410205, Hunan, China.

Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, 410004, China.

出版信息

BMC Microbiol. 2021 Mar 6;21(1):75. doi: 10.1186/s12866-021-02131-3.

OBJECTIVE

The aim of this study is to evaluate the efficacy of the strain Paenibacillus polymyxa HX-140, isolated from the rhizosphere soil of rape, to control Fusarium wilt of cucumber seedlings caused by Fusarium oxysporum f. sp. cucumerinum.

RESULTS

Strain HX-140 was able to produce protease, cellulase, β-1,3-glucanase and antifungal volatile organic compounds. An in vitro dual culture test showed that strain HX-140 exhibited broad spectrum antifungal activity against soil-borne plant pathogenic fungi. Strain HX-140 also reduced the infection of Fusarium wilt of cucumber seedlings by 55.6% in a greenhouse pot experiment. A field plot experiment confirmed the biocontrol effects and further revealed that antifungal activity was positively correlated with inoculum size by the root-irrigation method. Here, inoculums at 10 10 and 10 cfu/mL of HX-140 bacterial suspension reduced the incidence of Fusarium wilt of cucumber seedling by 19.5, 41.1, and 50.9%, respectively.

CONCLUSIONS

Taken together, our results suggest that P. polymyxa HX-140 has significant potential in the control of Fusarium wilt and possibly other fungal diseases of cucumber.

目的

本研究旨在评估从油菜根际土壤中分离得到的多粘类芽孢杆菌菌株 HX-140 对黄瓜幼苗枯萎病的防治效果，该病害由尖孢镰刀菌黄瓜专化型引起。

结果

菌株 HX-140 能够产生蛋白酶、纤维素酶、β-1,3-葡聚糖酶和抗真菌挥发性有机化合物。体外双培养试验表明，菌株 HX-140 对土壤传播的植物病原真菌具有广谱的抑菌活性。温室盆栽试验表明，菌株 HX-140 还将黄瓜枯萎病的感染率降低了 55.6%。田间小区试验证实了其生防效果，并进一步表明通过根灌法接种，抑菌活性与接种量呈正相关。在此条件下，10 10 和 10 cfu/mL 的 HX-140 细菌悬浮液接种可分别将黄瓜幼苗枯萎病的发病率降低 19.5%、41.1%和 50.9%。