Eco-Friendly Biomass Production and Identification of Active Compounds of EB.KN35 with Potent Anti- Effect.

is a fungal plant pathogen for over 100 agricultural crop species. There are strategies for managing wilt, including antagonistic bacteria that offer a promising and sustainable effect. In this work, among the various endophytic bacterial strains, EB.KN35 was selected as the best antifungal strain against . For eco-friendly biomass production of this bacterium, some agricultural byproducts were tested for cultivation, and a soybean processing byproduct (SPBP) was found to be a suitable C/N source for EB.KN35 fermentation. The utilization of a 14 L bioreactor system for EB.KN35 fermentation achieved a high biomass productivity (3.46 × 10 CFU/mL) in a short time (8 h). In bioactive compound analysis, EB.KN35 was found to be secreting several plant growth-promoting compounds such as GA3, IAA, kinetin, and zeatin (via HPLC) and eleven volatile compounds (via GC-MS). The docking study indicated that some volatile compounds (1, 2, 4, and 9) may play a significant role in inhibiting . The study results highlight the potential for reusing a soybean processing byproduct as a C/N source for the bioproduction of EB.KN35 with potential use as a biocontrol agent and biofertilizer.