Ghayyem Sena, Swaidan Abir, Barras Alexandre, Dolci Mathias, Faridbod Farnoush, Szunerits Sabine, Boukherroub Rabah
Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520, IEMN, Lille, F-59000, France; Analytical Chemistry Department, School of Chemistry, College of Science, University of Tehran, Tehran, Iran.
Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520, IEMN, Lille, F-59000, France.
Talanta. 2021 May 1;226:122082. doi: 10.1016/j.talanta.2021.122082. Epub 2021 Jan 6.
This paper reports on enzyme-like catalytic properties of polyethylene glycol-functionalized poly(N-phenylglycine) (PNPG-PEG) nanoparticles, which have not been explored to date. The developed nanoparticles have the ability to display great inherent peroxidase-like activity at very low concentrations, and are able to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) substrate in presence of hydrogen peroxide (HO). The oxidized product of TMB has a deep blue color with a maximum absorbance at ~655 nm. The PNPG-PEG nanoparticles exhibit K values of 0.2828 for TMB and 0.0799 for HO, indicating that TMB oxidation takes place at lower concentration of HO in comparison to other nanozymes. Based on the known mechanism of HO oxidation by hexavalent chromium [Cr(VI)] ions to generate hydroxyl radicals (OH), these nanoparticles were successfully applied for the colorimetric sensing of Cr(VI) ions. The sensor achieved good performance for Cr(VI) sensing with detection limits of 0.012 μM (0.01-0.1 μM linear range) and 0.52 μM (0.05-12.5 μM linear range). The detection scheme was highly selective, and successfully applied for the detection of Cr(VI) in real water samples.
本文报道了聚乙二醇功能化聚（N-苯基甘氨酸）（PNPG-PEG）纳米颗粒的类酶催化特性，这是迄今为止尚未探索过的。所开发的纳米颗粒能够在极低浓度下展现出很强的固有过氧化物酶样活性，并且能够在过氧化氢（HO）存在的情况下催化3,3',5,5'-四甲基联苯胺（TMB）底物的氧化。TMB的氧化产物呈深蓝色，在约655nm处有最大吸光度。PNPG-PEG纳米颗粒对TMB的K值为0.2828，对HO的K值为0.0799，这表明与其他纳米酶相比，TMB氧化在较低浓度的HO下发生。基于六价铬[Cr(VI)]离子氧化HO生成羟基自由基（OH）的已知机制，这些纳米颗粒成功应用于Cr(VI)离子的比色传感。该传感器对Cr(VI)传感具有良好性能，检测限为0.012μM（线性范围0.01 - 0.1μM）和0.52μM（线性范围0.05 - 12.5μM）。该检测方案具有高度选择性，并成功应用于实际水样中Cr(VI)的检测。
