Rostami Simindokht, Mehdinia Ali, Jabbari Ali
Department of Analytical Chemistry, Faculty of Chemistry, K. N. Toosi University of Technology, Tehran, Iran.
Department of Marine Living Science, Ocean Sciences Research Center, Iranian National Institute for Oceanography and Atmospheric Science, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2020 Sep;114:111034. doi: 10.1016/j.msec.2020.111034. Epub 2020 May 1.
Graphene nanoribbons (GNR) with intrinsic peroxidase mimic activity were introduced as a nanozyme with catalytic activity in oxidation of a typical chromogenic peroxidase substrate, 3,3',5,5'-tetramethylbenzidine (TMB), in the presence of HO. The proposed artificial enzyme was used as a label-free biosensor for rapid and sensitive colorimetric detection of dopamine (DA) according to a blue color fading which occurred due to the inhibition of TMB oxidation. Attractively, GNR exhibited an excellent catalytic activity over other carbon-based nanostructures due to its unique and extraordinary structural properties. Considering the changes of A versus DA concentration, a good linear dependency was achieved in the concentration range of 0.1-50 μM (0.1-1 and 2.5-50 μM) with a detection limit of 0.035 μM. The present peroxidase mimetic as a simple and rapid label-free sensor was successfully applied for detection of DA in serum samples, suggesting a promising sensitive sensing platform with great potential for biological and diagnostic applications.
具有内在过氧化物酶模拟活性的石墨烯纳米带（GNR）被引入作为一种纳米酶，在HO存在的情况下，对典型的显色过氧化物酶底物3,3',5,5'-四甲基联苯胺（TMB）的氧化具有催化活性。根据TMB氧化受到抑制而导致的蓝色褪色现象，所提出的人工酶被用作无标记生物传感器，用于快速、灵敏地比色检测多巴胺（DA）。吸引人的是，由于其独特而非凡的结构特性，GNR比其他碳基纳米结构表现出优异的催化活性。考虑到吸光度（A）与DA浓度的变化，在0.1 - 50 μM（0.1 - 1 μM和2.5 - 50 μM）的浓度范围内实现了良好的线性相关性，检测限为0.035 μM。这种过氧化物酶模拟物作为一种简单快速的无标记传感器，已成功应用于血清样品中DA的检测，表明其是一个具有很大生物和诊断应用潜力的有前景的灵敏传感平台。
