Department of Analytical Chemistry, Institute of Chemistry, Faculty of Science, University of Pavol Jozef Šafárik in Košice, Moyzesova 11, 040 01, Košice, Slovak Republic.
Department of Analytical Chemistry, Institute of Chemistry, Faculty of Science, University of Pavol Jozef Šafárik in Košice, Moyzesova 11, 040 01, Košice, Slovak Republic.
Talanta. 2021 May 1;226:122185. doi: 10.1016/j.talanta.2021.122185. Epub 2021 Feb 5.
A new and fully automated system with the interconnection of an Optical Immersion Probe (OIP) - pH meter - peristaltic pump was used to study the spectral and protolytic properties of carbocyanine the dyes 1,1',3,3,3',3'-hexamethylindocarbocyanine chloride (HIC); 1,1',3,3,3',3'-hexamethylindodicarbocyanine iodide (HIDC); and 3,3'-diethyloxadicarbocyanine iodide (DODC). This system can measure a large number of experimental points in a short time period. The effect of 32 various organic solvents on the UV-ViS spectra of the dyes was studied. The solvatochromic behaviour of studied dyes was characterized by positive solvatochromism for HIDC and negative solvatochromism for HIC and DODC. Through the application of a large number of experimental points, the protonation and hydrolysis constants of dyes were determined with high precision, where the confidence interval of the рK values is ±(0.001-0.005), compared with a confidence interval of ±(0.04-0.10) for standard procedures. The fully automated system presented is accurate, fast, environmentally friendly and promising for multiple analytical applications.
一个新的、完全自动化的系统，通过连接一个光学浸入探头 (OIP) - pH 计 - 蠕动泵，用于研究碳菁染料 1,1',3,3,3',3'-六甲基吲哚羰花青氯化物 (HIC)、1,1',3,3,3',3'-六甲基吲哚羰花青碘化物 (HIDC) 和 3,3'-二乙氧基羰花青碘化物 (DODC) 的光谱和质子化性质。该系统可以在短时间内测量大量实验点。研究了 32 种不同有机溶剂对染料紫外可见光谱的影响。研究染料的溶剂化行为表现为 HIDC 的正溶剂化变色和 HIC 和 DODC 的负溶剂化变色。通过应用大量的实验点，可以高精度地确定染料的质子化和解离常数，其中 рK 值的置信区间为 ±(0.001-0.005)，而标准程序的置信区间为 ±(0.04-0.10)。所提出的全自动系统准确、快速、环保，有望应用于多种分析应用。
