Department of Agricultural Biotechnology, Seoul National University, 1 Gwanakro, Gwanakgu, Seoul 08826, Republic of Korea.
Center for Food and Bioconvergence, Seoul National University, 1 Gwanakro, Gwanakgu, Seoul 08826, Republic of Korea.
Food Chem. 2021 Aug 1;352:129354. doi: 10.1016/j.foodchem.2021.129354. Epub 2021 Feb 20.
Biosensors have been widely applied in tests for allergens, but on-site detection remains a challenge. Herein, we proposed a detection procedure for peanut Ara h 1 as a representative allergen, which was extracted from a cookie, thereby minimising the need for any complex pretreatment that was difficult to perform, and enabling the visual detection of the target without the use of analytical equipment. The extraction procedure was performed in less than 30 min using a syringe and filter (0.45 μm). The detection method for Ara h 1 was based on the aggregation of switchable linkers (SL) and gold nanoparticles (AuNP), and the presence of 0.19 mg peanut protein per 30 g of cookie could be confirmed within 30 min based on the AuNP/SL concentration ratio by the precipitation. This proposed procedure could be successfully applied to the detection of a wide range of food allergens.
生物传感器已广泛应用于过敏原检测，但现场检测仍然是一个挑战。在此，我们提出了一种从曲奇中提取代表性过敏原花生 Ara h 1 的检测方法，从而最大限度地减少了对任何难以进行的复杂预处理的需求，并能够在无需使用分析设备的情况下对目标进行可视化检测。提取过程使用注射器和过滤器（0.45μm）在不到 30 分钟内完成。Ara h 1 的检测方法基于可切换连接物（SL）和金纳米粒子（AuNP）的聚集，并且可以根据沉淀的 AuNP/SL 浓度比在 30 分钟内确认每 30 克曲奇中存在 0.19 毫克花生蛋白。该方法可成功应用于多种食物过敏原的检测。
