Taihe Hospital, Hubei University of Medicine, Shiyan, 44200, Hubei, China.
Shiyan Center for Disease Control and Prevention, Shiyan, 442000, Hubei, China.
Environ Sci Pollut Res Int. 2021 Jul;28(27):35822-35829. doi: 10.1007/s11356-021-13247-4. Epub 2021 Mar 6.
This study aims to investigate the potential safety hazards and provide reference for improving the medical waste disposal procedure in SARS-CoV-2 testing laboratory. Our SARS-CoV-2 testing group detected the RNA residue on the surface of medical waste with Droplet Digital PCR, and held a meeting to discuss the risks in the laboratory medical waste disposal process. After effective autoclaving, SARS-CoV-2 contaminated on the surface of medical waste bags was killed, but the average concentration of viral RNA residues was still 0.85 copies/cm. It would not pose a health risk, but might contaminate the laboratory and affect the test results. When the sterilized medical waste bags were transferred directly by the operators without hand disinfection, re-contamination would happen, which might cause the virus to leak out of the laboratory. Furthermore, we found that sterilization effect monitoring and cooperation among operators were also very important. In summary, we investigated and analyzed the potential safety hazards during the medical waste disposal process in SARS-CoV-2 testing laboratory, and provided reasonable suggestions to ensure the safety of medical waste disposal.
本研究旨在探讨 SARS-CoV-2 检测实验室医疗废物处理程序中存在的潜在安全隐患,为改进医疗废物处理程序提供参考。我们的 SARS-CoV-2 检测组使用液滴数字 PCR 技术检测医疗废物表面的 RNA 残留,随后召开会议讨论实验室医疗废物处理过程中的风险。经过有效的高压蒸汽灭菌后,医疗废物袋表面污染的 SARS-CoV-2 被杀死,但病毒 RNA 残留的平均浓度仍为 0.85 拷贝/cm。这不会对健康造成危害,但可能会污染实验室并影响检测结果。当操作人员在未进行手部消毒的情况下直接转移已消毒的医疗废物袋时,就会发生再次污染,这可能导致病毒从实验室泄漏。此外,我们还发现,灭菌效果监测和操作人员之间的合作也非常重要。综上所述,我们对 SARS-CoV-2 检测实验室医疗废物处理过程中的潜在安全隐患进行了调查和分析,并提出了合理的建议,以确保医疗废物处理的安全性。