Floral and Nursery Plants Research Unit, US National Arboretum, USDA-ARS, Beltsville, MD, USA.
Biotechnology Career Academy, Applications and Research Laboratory, Ellicott City, MD, USA.
Arch Virol. 2021 May;166(5):1501-1505. doi: 10.1007/s00705-021-04999-y. Epub 2021 Mar 7.
The genus Carlavirus (family Betaflexiviridae, order Tymovirales) currently includes 53 species recognized by the ICTV. The NCBI GenBank database has 43 full-length carlavirus genome sequences (7,890 to 9,073 nt). Surprisingly, the type species Carnation latent virus is not associated with a complete genome sequence of a carnation latent virus (CLV) isolate; GenBank only has accessions with 1313 or fewer nucleotides. The goal of this study was to determine the full-length genome sequence of CLV. Naturally infected greenhouse-grown 'Kiwi Lace' carnation plants that tested positive for CLV by ELISA and RT-PCR were used as source plants for high-throughput sequencing, completed by 5' and 3' RACE and validated by Sanger sequencing of CLV-specific RT-PCR-generated amplicons. The complete CLV-KL sequence (MN450069) was determined to be 8,513 nt in length. In pairwise analysis, the genome shares 40-46% identity with recognized carlaviruses and the six in silico-translated proteins have 15-62% amino acid sequence identity to their respective carlavirus orthologs. The CLV-KL coat protein shares 98.4% identity with the NCBI reference sequence CLV-UK. In phylogenetic analysis, CLV clusters with butterbur mosaic virus, coleus vein necrosis virus, and garlic common latent virus. This is the first report of the full genome sequence of an isolate of carnation latent virus, the type member of the genus Carlavirus.
卡尔病毒属（贝塔柔链病毒科，Tymovirales 目）目前包括 53 种由 ICTV 认可的物种。NCBI GenBank 数据库中有 43 个完整的卡尔病毒基因组序列（7890 到 9073 个核苷酸）。令人惊讶的是，模式种康乃馨潜隐病毒与康乃馨潜隐病毒（CLV）分离物的完整基因组序列无关；GenBank 仅具有 1313 个或更少核苷酸的登录号。本研究的目的是确定 CLV 的全长基因组序列。通过 ELISA 和 RT-PCR 检测为阳性的自然感染温室生长的‘Kiwi Lace’康乃馨植物被用作高通量测序的源植物，通过 5' 和 3' RACE 完成，并通过 CLV 特异性 RT-PCR 扩增子的 Sanger 测序进行验证。确定完整的 CLV-KL 序列（MN450069）长度为 8513 个核苷酸。在成对分析中，基因组与已识别的卡尔病毒具有 40-46%的同一性，并且六个计算机翻译的蛋白质与各自的卡尔病毒同源物具有 15-62%的氨基酸序列同一性。CLV-KL 外壳蛋白与 NCBI 参考序列 CLV-UK 具有 98.4%的同一性。在系统发育分析中，CLV 与毛地黄花叶病毒、长春花脉坏死病毒和大蒜普通潜隐病毒聚类。这是第一个关于康乃馨潜隐病毒分离物全长基因组序列的报告，康乃馨潜隐病毒是卡尔病毒属的模式成员。
