Departamento de Ciências Farmacêuticas, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, CEP 23897-000 Seropédica, RJ, Brazil; Programa de Pós-graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, CEP 23897-000 Seropédica, RJ, Brazil.
Programa de Pós-graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, CEP 23897-000 Seropédica, RJ, Brazil; Departamento de Epidemiologia e Saúde Pública, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, CEP 23897-000 Seropédica, RJ, Brazil.
Vet Parasitol Reg Stud Reports. 2021 Jan;23:100515. doi: 10.1016/j.vprsr.2020.100515. Epub 2020 Dec 13.
Stomoxys calcitrans is a cosmopolitan hematophagous insect with significant veterinary importance. Besides causing great stress with its bites, the fly is a mechanical vector of several pathogens of the most diverse phylogenetic groups to their hosts. The objective of this study was to test for the presence of Anaplasma marginale in stable flies collected at three points inside the campus of the Federal Rural University of Rio de Janeiro (UFRRJ), Seropédica, Rio de Janeiro, Brazil. The collected flies were dissected and separated into three parts - heads, carcass and guts - which were individually submitted to A. marginale specific semi nested PCR gene amplification. A total 150 samples of 50 flies were assayed and 11.33% were positive with predominant presence in guts. In parallel, 6 F1 colony S. calcitrans flies fed with bovine blood were also investigated, being all positive for the presence of the bacteria. This is the first report of the presence of A. marginale in S. calcitrans at Rio de Janeiro state, actually in Brazil, indicating that the epidemiological importance of this vector in the transmission of diseases with great economic impact must not be ignored.
厩螫蝇是一种具有世界性分布的吸血昆虫，具有重要的兽医意义。除了叮咬会给动物带来巨大的压力外，这种蝇还是许多不同系统发育群病原体的机械传播媒介，会将病原体传播给宿主。本研究的目的是检测在巴西里约热内卢联邦农村大学（UFRRJ）塞罗佩迪卡校区内三个地点采集的厩螫蝇体内是否存在边缘无形体。采集的苍蝇被解剖并分为三个部分——头部、躯体和内脏——分别进行边缘无形体特异性半巢式 PCR 基因扩增。共检测了 150 个 50 只苍蝇样本，有 11.33%呈阳性，主要存在于内脏中。同时，还对 6 只用牛血喂养的 F1 品系厩螫蝇进行了检测，这些苍蝇均检测到了细菌的存在。这是在巴西里约热内卢州首次报告厩螫蝇体内存在边缘无形体，表明这种媒介在传播具有重大经济影响的疾病方面的流行病学重要性不容忽视。
