Tian Junhua, Liu Jing, Lu Miao, Chen Xiaomin, Li Kun
Wuhan Center for Disease Control and Prevention, 430024, Wuhan City, Hubei Province, China.
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Changping District, Beijing City, China.
Microb Pathog. 2023 Oct;183:106318. doi: 10.1016/j.micpath.2023.106318. Epub 2023 Aug 22.
Tabanids and stomoxes are important mechanical vectors for the transmission of pathogens. Although the agents they transmitted have been well studied, bacteria of the genus Anaplasma harbored by these flies have never been reported in China. In this study, 262 blood-sucking flies (128 Stomoxys calcitrans, 45 Tabanus birmanicus, 69 Tabanus hypomacros, and 20 Tabanus taiwanus) were collected from the Wuhan and Nanping cities of China. Anaplasma marginale, Anaplasma bovis, and Candidatus Anaplasma cinensis are detected in S. calcitrans from Wuhan City, with positive rates of 15.63%, 1.56%, and 7.81%, respectively. Out of our expectations, a putative novel Anaplasma species was identified in all three tabanid species (40.00% in T. birmanicus, 15.94% in T. hypomacros, and 10.00% in T. taiwanus) from Nanping City. The 16 S rRNA and groEL gene sequences have highest 99.37-99.75% and 91.46% identities to A. marginale, while the gltA gene sequences have highest 88.34% identity to Anaplasma centrale. In the phylogenetic trees, these strains form a distinct clade. Herein we name it "Candidatus Anaplasma nanpingensis". The present study shows the existence of multiple Anaplasma species in blood-sucking flies in China. This may be the first report that blood-sucking flies harbor Anaplasma in China.
虻类和厩螫蝇是病原体传播的重要机械性传播媒介。尽管它们传播的病原体已得到充分研究，但中国从未报道过这些蝇类携带的无形体属细菌。在本研究中，从中国武汉和南平两市采集了262只吸血蝇（128只厩螫蝇、45只中华斑虻、69只巨斑虻和20只台湾斑虻）。在武汉市采集的厩螫蝇中检测到边缘无形体、牛无形体和中华无形体，阳性率分别为15.63%、1.56%和7.81%。出乎我们意料的是，在南平的所有三种虻类中均鉴定出一种假定的新型无形体物种（中华斑虻中为40.00%，巨斑虻中为15.94%，台湾斑虻中为10.00%）。16S rRNA和groEL基因序列与边缘无形体的同源性最高，为99.37 - 99.75%，而gltA基因序列与中央无形体的同源性最高，为88.34%。在系统发育树中，这些菌株形成一个独特的分支。在此我们将其命名为“中华南平无形体（Candidatus Anaplasma nanpingensis）”。本研究表明中国吸血蝇中存在多种无形体物种。这可能是中国首次报道吸血蝇携带无形体。
