Florencio Melissa, Rosa Dayana, Riguete de Araújo Lima Karina, Araujo da Costa Gabriel, Vieira Guimarães Guedes Karine, Fampa Patrícia
Departamento de Ciências Farmacêuticas, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica- RJ 23.890-000, Brazil; Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica- RJ 23.890-000, Brazil.
Departamento de Ciências Farmacêuticas, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica- RJ 23.890-000, Brazil.
Vet Parasitol Reg Stud Reports. 2020 Jul;21:100434. doi: 10.1016/j.vprsr.2020.100434. Epub 2020 Jul 11.
Functional insect vector colonies are essential for the study of their biology, evolution, behavior as well as control strategies of these organisms that transmit pathogens of medical and veterinary importance. In addition to the establishment, improvement and adaptation of pre-established protocols in the maintenance of a colony is important, as it may result in higher production of insects. Stomoxys calcitrans or stable fly causes important economic losses in production of beef and milk, besides mechanically transmitting a great variety of pathogens to cattle. In order to generate flies under laboratory conditions, a colony of S. calcitrans was established at our laboratory considering local environment geographical characteristics. Adults collected in UFRRJ campus were kept in the laboratory and maintained with daily feeding on anticoagulant-containing bovine blood offered in disposable sanitary napkins. Immature forms were maintained on diets rich in organic matter. The colony temperature and relative humidity were daily monitored as well as the development of insects. The monthly measured biological parameters included the development of eggs into pupae, of pupae into F1 adults and the comparison of the number of flies collected in the field with the number of F1 flies emerged in the laboratory. The data presented here was obtained from March to November 2019. In that period, the average per month of flies collected in the field was 604.2, of generated eggs was 2694.3, of generated pupae was 349.5 and of generated F1 flies in the laboratory was 205.9. Fluctuations of abiotic and biological factors contributed to an uneven production of flies in the colony throughout the months. This is the first report with numerical quantification of S. calcitrans flies production in a laboratory in Brazil. Some methodologies used in the colony shall be reevaluated and modified with the goal of obtaining higher numbers of flies generated in the laboratory over time.
功能性昆虫媒介种群对于研究其生物学、进化、行为以及控制这些传播具有医学和兽医学重要性病原体的生物体的策略至关重要。除了建立、改进和调整既定的种群维持方案外，维持种群的重要性还在于它可能导致更高的昆虫产量。厩螫蝇会给牛肉和牛奶生产造成重大经济损失，此外还会将多种病原体机械传播给牛。为了在实验室条件下培育厩螫蝇，我们根据当地环境地理特征在实验室建立了一个厩螫蝇种群。在UF-RRJ校园收集的成虫被饲养在实验室中，每天用一次性卫生巾提供的含抗凝剂的牛血喂养。未成熟阶段则以富含有机物的食物饲养。每天监测种群的温度、相对湿度以及昆虫的发育情况。每月测量的生物学参数包括卵发育成蛹、蛹发育成F1代成虫，以及比较野外收集的苍蝇数量与实验室羽化的F1代苍蝇数量。这里呈现的数据是2019年3月至11月期间获得的。在那段时间里，每月野外收集的苍蝇平均数量为604.2只，产出的卵为2694.3个，产出的蛹为349.5个，实验室中羽化的F1代苍蝇为205.9只。非生物和生物因素的波动导致了整个月种群中苍蝇产量的不均衡。这是巴西一家实验室对厩螫蝇产量进行数值量化的首份报告。随着时间的推移，为了在实验室获得更多的苍蝇，种群中使用的一些方法应重新评估和改进。
