Elite Regenerative Stem Cell Specialists, LLC, Johnstown, Colorado, USA; R&D Regenerative Laboratory Resources, LLC, Johnstown, Colorado, USA.
Elite Regenerative Stem Cell Specialists, LLC, Johnstown, Colorado, USA; R&D Regenerative Laboratory Resources, LLC, Johnstown, Colorado, USA.
Cytotherapy. 2021 Aug;23(8):677-682. doi: 10.1016/j.jcyt.2021.01.004. Epub 2021 Mar 5.
Platelet-rich plasma (PRP) and bone marrow aspirate are commonly used in orthobiologics for their anti-inflammatory, anabolic/regenerative and immunomodulatory characteristics via platelet degranulation and cell secretions. Although platelets are derived from megakaryocytes in the bone marrow, no attention has been paid to the potential benefits of bone marrow platelets and whether their contents differ from aging platelets in peripheral blood.
In the present study, leukocyte-poor peripheral blood-derived platelets in plasma (LPP) and leukocyte-poor bone marrow platelets in plasma (BMP) were prepared from six donors, activated with calcium chloride, incubated and sampled at day 0, day 3 and day 6. LPP and BMP are platelet preparations intended to evaluate the respective platelet secretomes but are not classified as conventional PRPs, as they are not concentrated to the extent necessary to meet the qualifying criteria. At each time point, 15 growth and immunomodulatory factors were quantitated in LPP and BMP: platelet-derived growth factor AA, basic fibroblast growth factor/fibroblast growth factor 2, granulocyte-macrophage colony-stimulating factor, hepatocyte growth factor, macrophage colony-stimulating factor, stem cell factor, vascular endothelial growth factor, tumor necrosis factor alpha, IL-1β, interferon gamma, IL-4, IL-10, IL-1 receptor antagonist protein, IL-12p40 and arginase-1.
The results illustrate that platelets derived from bone marrow have a unique secretome profile compared with those derived from peripheral blood, with significant differences in anti-inflammatory cytokines, which are associated with monocyte polarization.
Ultimately, bone marrow-derived platelets may be useful as a stand-alone orthobiologic or as an effective adjuvant to autologous cell therapies where anti-inflammatory and anabolic processes are desired, especially with respect to monocyte function.
富血小板血浆（PRP）和骨髓抽吸物因其通过血小板脱颗粒和细胞分泌具有抗炎、合成代谢/再生和免疫调节特性而在骨科生物制剂中广泛应用。尽管血小板来源于骨髓中的巨核细胞，但尚未关注骨髓血小板的潜在益处，以及其内容是否与外周血中老化的血小板不同。
在本研究中，从六名供体中制备富含白细胞的血浆中的外周血来源血小板（LPP）和富含白细胞的骨髓血小板在血浆（BMP），用氯化钙激活，在第 0 天、第 3 天和第 6 天孵育和取样。LPP 和 BMP 是用于评估各自血小板分泌组的血小板制剂，但不被归类为常规 PRP，因为它们没有浓缩到足以满足合格标准的程度。在每个时间点，在 LPP 和 BMP 中定量了 15 种生长和免疫调节因子：血小板衍生生长因子 AA、碱性成纤维细胞生长因子/成纤维细胞生长因子 2、粒细胞-巨噬细胞集落刺激因子、肝细胞生长因子、巨噬细胞集落刺激因子、干细胞因子、血管内皮生长因子、肿瘤坏死因子-α、IL-1β、干扰素γ、IL-4、IL-10、IL-1 受体拮抗剂蛋白、IL-12p40 和精氨酸酶-1。
结果表明，与外周血来源的血小板相比，骨髓来源的血小板具有独特的分泌组特征，抗炎细胞因子存在显著差异，这与单核细胞极化有关。
最终，骨髓来源的血小板可用作独立的骨科生物制剂，或作为自体细胞治疗的有效佐剂，特别是在需要抗炎和合成代谢过程的情况下，尤其是在单核细胞功能方面。
