Van Oostveldt P, Boeken G
J Histochem Cytochem. 1977 Dec;25(12):1337-44. doi: 10.1177/25.12.336786.
Methods in use for the choice of the two-wavelengths and the effect of a faulty choice of the two wavelengths in the two-wavelength cytophotometric method of Patau and Ornstein are studied by means of a computer simulation and in practice upon pararosaniline-Schiff stained nuclei. A simplification of the measuring strategy is proposed without affecting the accuracy.
通过计算机模拟以及在对副品红-席夫染色细胞核的实际操作中,研究了帕陶和奥恩斯坦双波长细胞光度法中选择两个波长的使用方法以及两个波长选择错误的影响。提出了一种测量策略的简化方法,且不影响准确性。