Van der Ploeg M, Van den Broek K, Smeulders A W, Vossepoel A M, Van Duijn P
Histochemistry. 1977 Dec 28;54(4):273-88. doi: 10.1007/BF00508271.
A description is given of a combination of three programs developed for computer-assisted stage scanning cytophotometry and cytofluorometry of isolated, close-lying, or touching objects in different types of microscopical preparations. The advantages and limitations of the individual programs are discussed, as well as the local specimen conditions determining the optimal application range of each of the programs. The applicability of these programs was investigated by determination of the integrated absorbance values of Feulgen or gallocyanin-chrome alum stained chicken erythrocytes, human leucocytes, and skin biopsy cells in imprint preparations, as well as of guinea pig peritoneal granulocytes which had been submitted to a simultaneous coupling azo dye incubation for alkaline phosphatase activity.
本文描述了为计算机辅助的阶段扫描细胞光度测定法和细胞荧光测定法开发的三个程序的组合，用于对不同类型显微镜标本中分离的、紧密相邻的或相互接触的物体进行分析。讨论了各个程序的优点和局限性，以及决定每个程序最佳应用范围的局部标本条件。通过测定福尔根或间苯二酚蓝-铬明矾染色的鸡红细胞、人白细胞和印记标本中的皮肤活检细胞的积分吸光度值，以及对豚鼠腹膜粒细胞进行碱性磷酸酶活性的偶氮染料同步孵育后，研究了这些程序的适用性。
