Rasch E M, Rasch R W
J Histochem Cytochem. 1979 Oct;27(10):1384-7. doi: 10.1177/27.10.92497.
Lack of appropriate software support for microprocessor program development has previously limited the applications of such technology in the field of microspectrophotometry. This paper describes our use of a Vickers M86 integrating microdensitometer coupled through a custom-designed interface circuit to a Processor Technology SOL III microcomputer. W e have developed a series of interactive, user-oriented programs for DNA-Feulgen cytophotometry with this instrument to allow automatic storage of data in files on floppy disks and instant retrieval of sets of measurements for statistical processing. The same data files can also be used to generate graphic displays in the form of bar histograms or plots of linear regressions on a video monitor and to produce hardcopy output of data files and graphic displays through the use of a high speed DIABLO printer.
先前，缺乏适用于微处理器程序开发的软件支持限制了此类技术在显微分光光度测定领域的应用。本文介绍了我们将一台通过定制接口电路与Processor Technology SOL III微型计算机相连的维氏M86积分光密度计的使用情况。我们用该仪器开发了一系列用于DNA-福尔根细胞光度测定的交互式、面向用户的程序，以便将数据自动存储在软盘文件中，并能即时检索测量数据集以进行统计处理。相同的数据文件还可用于在视频监视器上生成条形直方图或线性回归图形式的图形显示，并通过使用高速DIABLO打印机输出数据文件和图形显示的硬拷贝。
