山竹提取物抑制白细胞介素 1β诱导的软骨细胞凋亡。
Mangiferin attenuates IL1βinduced chondrocytes apoptosis.
机构信息
Department of Orthopedics, Second Affiliated Hospital of Xi'an Medical University, Xi'an 710038, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021 Jan 28;46(1):25-31. doi: 10.11817/j.issn.1672-7347.2021.190287.
OBJECTIVES
Chondrocyte apoptosis is an important process in the pathogenesis of osteoarthritis. Mangiferin exerts multiple pharmacological effects such as anti-inflammatory and anti-apoptosis. However, the role of mangiferin in chondrocyte apoptosis is not clear. In this study, we aimed to explore the role of mangiferin in IL-1β-induced chondrocyte apoptosis.
METHODS
ATDC5 cells were randomly divided into a control group, a IL-1β group, a MFN-L group, a MFN-M group, a MFN-H group and a MFN+LY294002 group. Cells in the control group were treated with IL-1β (10 ng/mL) for 24 h; cells in the MFN-L group, the MFN-M group and the MFN-H group were pretreated with 5, 10 and 20 μmol/L mangiferin for 1 h respectively, and then they were treated with IL-1β (10 ng/mL) for 24 h; cells in the MFN+LY294002 group were treated with LY294002 (25 μmol/L) for 1 h, then mangiferin (20 μmol/L) and IL-1β (10 ng/mL) for 1 h and 24 h, respectively. Cell viability was detected by CCK-8 assay and cell apoptosis was measured by flow cytometry. Colorimetric assay was conducted to measure the caspase-3 activity. The protein levels of Bcl-2, Bax, and phosphoinositide 3-kinase (PI3K)/Akt signaling pathway related proteins were detected by Western blotting.
RESULTS
Compared to the control group, cell viability was significantly decreased; cell apoptosis, caspase-3 activity and Bax protein expression were significantly increased; the protein levels of Bcl-2, p-PI3K, and p-Akt were significantly decreased in the IL-1β group (all <0.05). Compared to the IL-1β group, cell viability was significantly increased; cell apoptosis, caspase-3 activity and Bax protein expression were significantly decreased; the protein levels of Bcl-2, p-PI3K, and p-Akt were significantly increased in the MFN-L, the MFN-M and the MFN-H groups (all <0.05). Compared to the MFN-M group, cell apoptosis and the protein level of Bax in the MFN+LY294002 group were significantly increased; the Bcl-2 protein expression was significantly decreased (all <0.05).
CONCLUSIONS
Mangiferin could attenuate IL-1β-induced apoptosis of the mice chondrocytes, which is mediated by the activation of PI3K/Akt signaling pathway.
目的
软骨细胞凋亡是骨关节炎发病机制中的一个重要过程。芒果苷具有抗炎和抗凋亡等多种药理作用。然而，芒果苷在软骨细胞凋亡中的作用尚不清楚。本研究旨在探讨芒果苷在 IL-1β诱导的软骨细胞凋亡中的作用。
方法
将 ATDC5 细胞随机分为对照组、IL-1β 组、MFN-L 组、MFN-M 组、MFN-H 组和 MFN+LY294002 组。对照组细胞用 10ng/mlIL-1β 处理 24h；MFN-L 组、MFN-M 组和 MFN-H 组分别用 5、10 和 20μmol/L 芒果苷预处理 1h，然后用 10ng/mlIL-1β 处理 24h；MFN+LY294002 组先用 25μmol/L LY294002 处理 1h，再用 20μmol/L 芒果苷和 10ng/mlIL-1β 分别处理 1h 和 24h。用 CCK-8 法检测细胞活力，用流式细胞术检测细胞凋亡。比色法检测 caspase-3 活性。Western blot 检测 Bcl-2、Bax 和磷酸肌醇 3-激酶（PI3K）/Akt 信号通路相关蛋白的水平。
结果
与对照组相比，IL-1β 组细胞活力显著降低，细胞凋亡、caspase-3 活性和 Bax 蛋白表达显著增加，Bcl-2、p-PI3K 和 p-Akt 蛋白水平显著降低（均<0.05）。与 IL-1β 组相比，MFN-L、MFN-M 和 MFN-H 组细胞活力显著增加，细胞凋亡、caspase-3 活性和 Bax 蛋白表达显著降低，Bcl-2、p-PI3K 和 p-Akt 蛋白水平显著升高（均<0.05）。与 MFN-M 组相比，MFN+LY294002 组细胞凋亡和 Bax 蛋白水平显著增加，Bcl-2 蛋白表达显著降低（均<0.05）。
结论
芒果苷可通过激活 PI3K/Akt 信号通路减轻 IL-1β 诱导的小鼠软骨细胞凋亡。
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