Hayati Farida, Chabib Lutfi, Fauzi It Silmi, Awaluddin Rizki, Faizah Wan Syarifah, Mohd Nasir Moh Hamzah, Nipun T S
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia, Ngemplak, Indonesia.
Department of Pharmacy, Faculty of Health Science, University of Darussalam Gontor, Indonesia.
J Pharm Bioallied Sci. 2020 Oct-Dec;12(4):457-461. doi: 10.4103/jpbs.JPBS_297_19. Epub 2020 Oct 8.
Pegagan is a traditional medicinal plant with three major bioactive properties, triterpenoid, steroids, and saponin. It has the properties of antioxidant, antistress, and wound healing. Pegagan extract is prepared in self-nanoemulsifying drug delivery systems (SNEDDS) to overcome the problem of low water-solubility level.
This study aimed to observe the effect of pegagan ethanolic extract SNEDDS on the development of zebrafish embryos.
This study used 12 sets of zebrafish embryos presented in five sets of extract SNEDDS with different concentrations, that is, 20, 10, 5, 2.5, and 1.25 μg, five sets of SNEDDS without extract with different concentrations, that is, 20, 10, 5, 2.5, and 1.25 μg, a set of positive control (3.4-DCA 4 mg/L) with one control set (diluted with water), and a negative control (SNEDDS without extract). The procedure was conducted for 96 h with observations every 24 h. The parameters observed were embryonic coagulation, formation of somites, detachment of tail bud from the yolk, and abnormality of embryo.
The results showed that in 96 h the 20ppm concentration caused 100% mortality. Embryo abnormality appeared as coagulation of embryo, somite malformation, and abnormal tail.
There is a correlation between the concentration of SNEDDS and the incidence of embryo coagulation. The malformation in the group of pegagan extract SNEDDS is characterized by cardiac edema, somite malformation, and abnormal tail.
Pegagan ethanolic extract SNEDDS of 20ppm can inhibit the development of zebrafish embryos.
雷公根是一种具有三种主要生物活性成分（三萜类、甾体类和皂苷类）的传统药用植物。它具有抗氧化、抗应激和伤口愈合的特性。雷公根提取物被制备成自纳米乳化药物递送系统（SNEDDS）以克服其低水溶性的问题。
本研究旨在观察雷公根乙醇提取物SNEDDS对斑马鱼胚胎发育的影响。
本研究使用了12组斑马鱼胚胎，分为五组不同浓度的提取物SNEDDS（即20、10、5、2.5和1.25微克），五组不同浓度的无提取物SNEDDS（即20、10、5、2.5和1.25微克），一组阳性对照（3,4 - 二氯苯胺4毫克/升）和一组对照组（用水稀释），以及一组阴性对照（无提取物的SNEDDS）。实验过程持续96小时，每24小时进行观察。观察的参数包括胚胎凝固、体节形成、尾芽与卵黄的分离以及胚胎异常。
结果表明，在96小时内，20ppm浓度导致100%死亡率。胚胎异常表现为胚胎凝固、体节畸形和尾巴异常。
SNEDDS的浓度与胚胎凝固的发生率之间存在相关性。雷公根提取物SNEDDS组的畸形特征为心脏水肿、体节畸形和尾巴异常。
20ppm的雷公根乙醇提取物SNEDDS可抑制斑马鱼胚胎的发育。
