Hayati Farida, Ramadani Arba P, Putri Cynthia A, Chabib Lutfi, Putri Farah U, Nugroho Agung E
Department of Pharmacy, Universitas Islam Indonesia, Sleman Regency, Special Region of Yogyakarta, Indonesia.
Faculty of Pharmacy, Universitas Gadjah Mada, Sleman Regency, Special Region of Yogyakarta, Indonesia.
J Pharm Bioallied Sci. 2024 Apr-Jun;16(2):51-59. doi: 10.4103/jpbs.jpbs_1139_23. Epub 2024 Jul 15.
The chronic diabetes mellitus (DM) condition may lead to diabetic wounds that increase morbidity in patients. Poir leaves have been widely reported to possess anti-diabetic activity due to their flavonoid contents. To enhance drug penetration, a nanoemulgel preparation was formulated.
This study aimed to evaluate the activity of nanoemulgel preparations of Poir leaf extract on diabetic and non-diabetic wound-healing using male Wistar rats.
This research was an experimental study with a post-test only control group design.
The rats (n = 32) were randomly divided into two groups: diabetic (induced by 40 mg/kg BW STZ) and non-diabetic model. Each model consisted of four groups: normal, positive control, leaf extract (IRLE), and nanoemulgel of leaf extract (NIRLE). All the animals studied were shaved from the back, and a 2.5 × 0.5 cm full-thickness excision wound was made. IRLE and NIRLE were administered daily and observed for the wound-healing process.
The one-way analysis of variance with the Tukey post-hoc test was used for the statistical analysis.
A NIRLE formulation has been developed to produce a preparation that meets the physical requirements. IRLE and NIRLE possessed wound-healing activity in normal and diabetic rat models. However, the wound-healing process in diabetic rats treated with NIRLE was faster than those with IRLE.
NIRLE increased the activity of wound-healing effect of leaves on diabetic rats in comparison with the extract form.
慢性糖尿病（DM）可能导致糖尿病伤口，增加患者的发病率。由于其黄酮类成分，宝树叶子已被广泛报道具有抗糖尿病活性。为了增强药物渗透，制备了纳米乳凝胶制剂。
本研究旨在使用雄性Wistar大鼠评估宝树叶子提取物纳米乳凝胶制剂对糖尿病和非糖尿病伤口愈合的活性。
本研究是一项仅设有后测对照组设计的实验研究。
将大鼠（n = 32）随机分为两组：糖尿病组（通过40 mg/kg体重的链脲佐菌素诱导）和非糖尿病模型组。每个模型组又分为四组：正常组、阳性对照组、叶子提取物组（IRLE）和叶子提取物纳米乳凝胶组（NIRLE）。对所有研究的动物进行背部脱毛，并制作一个2.5×0.5 cm的全层切除伤口。每天给予IRLE和NIRLE，并观察伤口愈合过程。
采用单因素方差分析和Tukey事后检验进行统计分析。
已开发出一种NIRLE制剂，以生产出符合物理要求的制剂。IRLE和NIRLE在正常和糖尿病大鼠模型中均具有伤口愈合活性。然而，用NIRLE治疗的糖尿病大鼠的伤口愈合过程比用IRLE治疗的大鼠更快。
与提取物形式相比，NIRLE增强了宝树叶子对糖尿病大鼠的伤口愈合作用。
