A Wound-Healing Effect of Nanoemulgel of Kangkung (, Poir) Leaf Extract in STZ Diabetic Rats.

CONTEXT

The chronic diabetes mellitus (DM) condition may lead to diabetic wounds that increase morbidity in patients. Poir leaves have been widely reported to possess anti-diabetic activity due to their flavonoid contents. To enhance drug penetration, a nanoemulgel preparation was formulated.

AIMS

This study aimed to evaluate the activity of nanoemulgel preparations of Poir leaf extract on diabetic and non-diabetic wound-healing using male Wistar rats.

SETTINGS AND DESIGN

This research was an experimental study with a post-test only control group design.

MATERIALS AND METHODS

The rats (n = 32) were randomly divided into two groups: diabetic (induced by 40 mg/kg BW STZ) and non-diabetic model. Each model consisted of four groups: normal, positive control, leaf extract (IRLE), and nanoemulgel of leaf extract (NIRLE). All the animals studied were shaved from the back, and a 2.5 × 0.5 cm full-thickness excision wound was made. IRLE and NIRLE were administered daily and observed for the wound-healing process.

STATISTICAL ANALYSIS USED

The one-way analysis of variance with the Tukey post-hoc test was used for the statistical analysis.

RESULTS

A NIRLE formulation has been developed to produce a preparation that meets the physical requirements. IRLE and NIRLE possessed wound-healing activity in normal and diabetic rat models. However, the wound-healing process in diabetic rats treated with NIRLE was faster than those with IRLE.

CONCLUSIONS

NIRLE increased the activity of wound-healing effect of leaves on diabetic rats in comparison with the extract form.