Sukmawan Yedy Purwandi, Alifiar Ilham, Nurdianti Lusi, Ningsih Widar Rahayu
Bakti Tunas Husada Health Science College, Department of Pharmacology and Clinical Pharmacy, Tasikmalaya, Indonesia
Bakti Tunas Husada Health Science College, Department of Pharmaceuthics, Tasikmalaya, Indonesia
Turk J Pharm Sci. 2021 Oct 28;18(5):609-615. doi: 10.4274/tjps.galenos.2021.34676.
The study's objective was to determine the wound healing activity of the combination of ethanolic extracts of L. leaf (white and purple), , and astaxanthin gel preparation.
For in-gel preparation, three different formulas of gelling agents, namely carbopol 934 (1%), hydroxypropyl methylcellulose (HPMC) (9%), and natirum-carboxymethylcellulose (Na-CMC) (4%), were employed. Then, the organoleptic, pH, spreadability, and viscosity of the formulas were evaluated. To determine wound healing activity, six treatments, including negative control (placebo), positive control (bioplacenton), BP5 ( L. leaf ethanolic extract of white flower type 5%, L. Urb leaf ethanolic extract 2.5%, astaxanthin 0.05%), BU5 ( L. leaf ethanolic extract of purple flower type 5%, L. Urb leaf ethanolic extract 2.5%, astaxanthin 0.05%), BU10 ( L. leaf ethanolic extract of purple flower type 10%, L. Urb leaf ethanolic extract 5%, and astaxanthin 0.1%), and BP10 ( L. leaf ethanolic extract of white flower type 10%, L. Urb leaf ethanolic extract 5%, and astaxanthin 0.1%) were evaluated. All treatments were applied to an incision wound (1.5 cm). Measurement of the wound length was conducted daily for 14 days.
The results showed that the carbopol 934 (1%) gelling agent formula was better than HPMC and Na-CMC. Meanwhile, the percentages of wound healing activity for negative, positive, BP5, BU5, BU10, and BP10 groups were 72.51%, 69.36%, 70.14%, 81.70%, 86.54%, and 80.21%, respectively. The BU5 and BU10 showed significant activity (p<0.05) compared with positive and negative controls.
BU10 provided the best wound healing activity and can be developed as a commercial product.
本研究的目的是确定白花和紫花的L.叶乙醇提取物与虾青素凝胶制剂组合的伤口愈合活性。
对于凝胶制剂，使用了三种不同配方的胶凝剂，即卡波姆934（1%）、羟丙基甲基纤维素（HPMC）（9%）和羧甲基纤维素钠（Na-CMC）（4%）。然后，对配方的感官特性、pH值、铺展性和粘度进行了评估。为了确定伤口愈合活性，评估了六种处理方法，包括阴性对照（安慰剂）、阳性对照（生物胎盘素）、BP5（白花型L.叶乙醇提取物5%、L. Urb叶乙醇提取物2.5%、虾青素0.05%）、BU5（紫花型L.叶乙醇提取物5%、L. Urb叶乙醇提取物2.5%、虾青素0.05%）、BU10（紫花型L.叶乙醇提取物10%、L. Urb叶乙醇提取物5%、虾青素0.1%）和BP10（白花型L.叶乙醇提取物10%、L. Urb叶乙醇提取物5%、虾青素0.1%）。所有处理均应用于一个1.5厘米的切口伤口。每天测量伤口长度，持续14天。
结果表明，卡波姆934（1%）胶凝剂配方优于HPMC和Na-CMC。同时，阴性、阳性、BP5、BU5、BU10和BP10组的伤口愈合活性百分比分别为72.51%、69.36%、70.14%、81.70%、86.54%和80.21%。与阳性和阴性对照相比，BU5和BU10表现出显著活性（p<0.05）。
BU10具有最佳的伤口愈合活性，可以开发成商业产品。
