硫酸二乙酯对人淋巴细胞培养物和V79中国仓鼠细胞中染色体数目畸变的诱导作用。

The induction of numerical chromosome aberrations in human lymphocyte cultures and V79 Chinese hamster cells by diethyl sulfate.

作者信息

De Ferrari M, Bonatti S, Pisano V, Viaggi S, Abbondandolo A

机构信息

Laboratory of Mutagenesis, IST, Genova, Italy.

出版信息

Mutat Res. 1988 May-Aug;205(1-4):409-14. doi: 10.1016/0165-1218(88)90031-6.

DOI:10.1016/0165-1218(88)90031-6

PMID:3367925

Abstract

Human lymphocyte cultures were treated with increasing concentrations of diethyl sulfate (DES) at different times after stimulation with phytohemagglutinin (0, 24 and 45 h) and were scored for numerical chromosomal aberrations at different culture times (52, 72 and 96 h). A total of 3500 metaphases were analyzed. A statistically significant (p less than 0.01) increase in hypodiploid and hyperdiploid metaphases was found throughout the tested dose range (0.1-3 mM DES); the increase in polyploid metaphases was statistically significant at 1 mM (p less than 0.05) and 3 mM (p less than 0.01) DES. In human lymphocytes treated in Go, DES also induced chromatid breaks as well as micronuclei. In V79 Chinese hamster cells, DES induced micronuclei and polyploidy.

摘要

用人淋巴细胞培养物在植物血凝素刺激后的不同时间（0、24和45小时）用浓度递增的硫酸二乙酯（DES）处理，并在不同培养时间（52、72和96小时）对染色体数目畸变进行评分。共分析了3500个中期分裂相。在整个测试剂量范围（0.1 - 3 mM DES）内，亚二倍体和超二倍体中期分裂相有统计学显著增加（p < 0.01）；在1 mM（p < 0.05）和3 mM（p < 0.01）DES时，多倍体中期分裂相的增加有统计学意义。在处于G0期处理的人淋巴细胞中，DES还诱导了染色单体断裂以及微核。在V79中国仓鼠细胞中，DES诱导了微核和多倍体。