Dana Nicholas, Fowler R Andrew, Allen Alicia, Zoldan Janet, Suggs Laura, Emelianov Stanislav
Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, 78712, USA.
School of Electrical & Computer Engineering and Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, Georgia, 30332, USA.
Laser Phys Lett. 2016 Jul;13(7). doi: 10.1088/1612-2011/13/7/075603. Epub 2016 Jun 10.
Imaging of cellular electric potential via calcium-ion sensitive contrast agents is a useful tool, but current it lacks sufficient depth penetration. We explore contrast-enhanced photoacoustic (PA) imaging, using Arsenazo III dye, to visualize cardiac myocyte depolarization . Phantom results show strong linearity of PA signal with dye concentration ( > 0.95), and agree spectrally with extinction measurements with varying calcium concentration. Cell studies indicate a significant (> 100-fold) increase in PA signal for dye-treated cells, as well as a 10-fold increase in peak-to-peak variation during a 30-second window. This suggests contrast-enhanced PA imaging may have sufficient sensitivity and specificity for depth-resolved visualization of tissue depolarization in real-time.
通过钙离子敏感造影剂对细胞电势进行成像，是一种有用的工具，但目前它缺乏足够的深度穿透能力。我们探索了使用偶氮胂III染料的对比增强光声（PA）成像，以可视化心肌细胞去极化。模型结果显示PA信号与染料浓度具有很强的线性关系（>0.95），并且在光谱上与不同钙浓度下的消光测量结果一致。细胞研究表明，经染料处理的细胞的PA信号显著增加（>100倍），并且在30秒的窗口内峰峰值变化增加了10倍。这表明对比增强PA成像可能具有足够的灵敏度和特异性，用于实时深度分辨组织去极化的可视化。
