Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton, Stop C0800, Austin, Texas 78712, USA.
ACS Nano. 2013 Feb 26;7(2):1272-80. doi: 10.1021/nn304739s. Epub 2013 Jan 17.
Quantitative visualization of nanoparticles in cells and tissues, while preserving the spatial information, is very challenging. A photoacoustic imaging technique to depict the presence and quantity of nanoparticles is presented. This technique is based on the dependence of the photoacoustic signal on both the nanoparticle quantity and the laser fluence. Quantitative photoacoustic imaging is a robust technique that does not require knowledge of the local fluence, but a relative change in the fluence. This eliminates the need for sophisticated methods or models to determine the energy distribution of light in turbid media. Quantitative photoacoustic imaging was first applied to nanoparticle-loaded cells, and quantitation was validated by inductively coupled plasma mass spectrometry. Quantitative photoacoustic imaging was then extended to xenograft tumor tissue sections, and excellent agreement with traditional histopathological analysis was demonstrated. Our results suggest that quantitative photoacoustic imaging may be used in many applications including the determination of the efficiency and effectiveness of molecular targeting strategies for cell studies and animal models, the quantitative assessment of photoacoustic contrast agent biodistribution, and the validation of in vivo photoacoustic imaging.
在保留空间信息的情况下，对细胞和组织中的纳米粒子进行定量可视化是非常具有挑战性的。本文提出了一种基于光声信号既依赖于纳米粒子数量又依赖于激光强度的方法来描述纳米粒子的存在和数量。定量光声成像是一种稳健的技术，不需要了解局部强度，只需要相对强度的变化。这消除了对复杂方法或模型的需求，以确定混浊介质中的光能量分布。定量光声成像首先应用于负载纳米粒子的细胞，并通过电感耦合等离子体质谱法进行定量验证。定量光声成像随后扩展到异种移植肿瘤组织切片，并与传统的组织病理学分析显示出极好的一致性。我们的结果表明，定量光声成像可能在许多应用中使用，包括确定细胞研究和动物模型中分子靶向策略的效率和有效性，定量评估光声造影剂的生物分布，以及验证体内光声成像。
