Wang Xingmin, Xu Jing, Sun Tingfei, Ali Shaukat
Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Research Centre of Biological Control, College of Plant protection, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2021 Feb 18;12:630220. doi: 10.3389/fmicb.2021.630220. eCollection 2021.
Nanotechnology can offer an environmentally sustainable alternative to synthetic chemicals for pest management. Nano-formulations of different microbial pest control agents have been effective against several insect pests. Synthesis of -biochar (BC) nanoparticles and their bio-efficacy against was observed during this study. The characterization of BC nanoparticles through different analytical techniques showed successful synthesis of nanoparticles. UV spectroscopy showed a characteristic band of surface plasmon between 350 and 400 nm; SEM images confirmed the synthesis of spherical shaped nanoparticles; X-ray diffractogram showed strong peaks between 2θ values of 20°-25°; and atomic force microscopy (AFM) analysis revealed particle size of 49.151 nm. The bioassay studies demonstrated that different concentrations of BC nanoparticles caused significant reduction in hatchability of eggs as well as survival of immatures emerging from treated eggs when compared with controls. The results also revealed that BC nanoparticles were highly pathogenic against 2nd and 3rd instar nymphs and pupae of having LC values of 6.80, 7.45, and 8.64 ppm, respectively. The LT values for 20 ppm concentration of BC nanoparticles against 2nd and 3rd instar nymphs, and pupae of were 3.25 ± 0.29, 3.69 ± 0.52, and 4.07 ± 0.51 days, respectively. These findings suggest that BC nanoparticles can potentially be used in biorational management programs.
纳米技术可为害虫管理提供一种环境可持续的合成化学物质替代品。不同微生物害虫防治剂的纳米制剂已对多种害虫有效。本研究期间观察到了生物炭(BC)纳米颗粒的合成及其对[害虫名称]的生物功效。通过不同分析技术对BC纳米颗粒进行表征,结果表明纳米颗粒成功合成。紫外光谱显示在350至400纳米之间有表面等离子体的特征带;扫描电子显微镜图像证实合成了球形纳米颗粒;X射线衍射图显示在2θ值为20° - 25°之间有强峰;原子力显微镜分析显示颗粒尺寸为49.151纳米。生物测定研究表明,与对照相比,不同浓度的BC纳米颗粒使[害虫名称]卵的孵化率以及处理后卵中羽化出的未成熟个体的存活率显著降低。结果还显示,BC纳米颗粒对[害虫名称]的2龄和3龄若虫及蛹具有高致病性,其LC值分别为6.80、7.45和8.64 ppm。20 ppm浓度的BC纳米颗粒对[害虫名称]2龄和3龄若虫及蛹的LT值分别为3.25 ± 0.29、3.69 ± 0.52和4.07 ± 0.51天。这些发现表明BC纳米颗粒有可能用于生物合理的[害虫名称]管理计划。