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铁纳米颗粒对法氏囊的毒性及生物学效应

Toxicity and Biological Effects of Fe Nanoparticles against (Fabricius).

作者信息

Xu Jing, Zhang Kaihui, Cuthbertson Andrew G S, Du Cailian, Ali Shaukat

机构信息

Key Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, China.

Engineering Research Center of Biological Control, Ministry of Education and Guangdong Province, South China Agricultural University, Guangzhou 510642, China.

出版信息

Insects. 2020 Dec 21;11(12):895. doi: 10.3390/insects11120895.

Abstract

Nanotechnology has clear potential in the development of innovative insecticidal products for the biorational management of major insect pests. Metal-based nanoparticles of different microbial pest control agents have been effective against several pests. Synthesis of based Fe nanoparticles (FeNPs) and their bio-efficacy against was observed during this study. conidia were coated with FeNPs and characterized by applying a selection of different analytical techniques. Ultraviolet (UV) spectroscopy showed the characteristic band of surface plasmon at 430 nm; Scanning electron microscopy (SEM) images showed spherical shaped nanoparticles with a size ranging between 0.41 to 0.80 µm; Energy-dispersive X-ray (EDX) spectral analysis revealed characteristic Fe peaks at 6.5 and 7.1 Kev; the X-ray diffractogram showed three strong peaks at 2 values of 45.72°, 64.47°, and 84.05°. The bioassay studies demonstrated that mortality of 2nd instar larvae following FeNPs treatment increased with increasing concentrations of FeNPs at different time intervals. The median lethal concentration (LC) values of FeNPs against after seven days of fungal treatment was 59 ppm, whereas median survival time (LT) values for 200 and 500 ppm concentrations of FeNPs against seven days post-treatment were 5.1 and 2.29 days, respectively. -FeNPs caused significant reductions in feeding and growth parameters (relative growth rate, relative consumption rate, and efficiency of conversion of ingested food) of . FeNPs induced reduction in glutathione-S-transferase activities throughout the infection period whereas activities of antioxidant enzymes decreased during later periods of infection. These findings suggest that FeNPs can potentially be used in biorational management programs.

摘要

纳米技术在开发用于主要害虫生物合理治理的创新杀虫产品方面具有明显潜力。不同微生物害虫防治剂的金属基纳米颗粒已对多种害虫有效。在本研究中观察到了基于[未提及具体物质]的铁纳米颗粒(FeNPs)的合成及其对[未提及具体害虫]的生物活性。[未提及具体害虫]的分生孢子用FeNPs包被,并通过应用多种不同分析技术进行表征。紫外(UV)光谱显示在430 nm处有表面等离子体的特征峰;扫描电子显微镜(SEM)图像显示为球形纳米颗粒,尺寸在0.41至0.80 µm之间;能量色散X射线(EDX)光谱分析揭示在6.5和7.1 Kev处有特征性的铁峰;X射线衍射图显示在2θ值为45.72°、64.47°和84.05°处有三个强峰。生物测定研究表明,在不同时间间隔,随着FeNPs浓度增加,经FeNPs处理的二龄[未提及具体害虫]幼虫死亡率升高。真菌处理七天后,FeNPs对[未提及具体害虫]的半数致死浓度(LC)值为59 ppm,而处理七天后,200和500 ppm浓度的FeNPs对[未提及具体害虫]的半数存活时间(LT)值分别为5.1天和2.29天。[未提及具体物质]-FeNPs导致[未提及具体害虫]的取食和生长参数(相对生长率、相对消耗率和摄入食物转化率)显著降低。在整个感染期,[未提及具体物质]-FeNPs诱导谷胱甘肽-S-转移酶活性降低,而抗氧化酶活性在感染后期下降。这些发现表明,[未提及具体物质]-FeNPs可潜在地用于生物合理的[未提及具体害虫]治理计划。

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