Medical Microbiology, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands.
GlaxoSmithKline Vaccines S.r.l., Siena, Italy.
Front Immunol. 2021 Feb 19;12:635825. doi: 10.3389/fimmu.2021.635825. eCollection 2021.
Neutrophils play a key role in the human immune response to infections. These professional phagocytes rapidly migrate to the site of infection to engulf bacteria and destroy them specialized intracellular killing mechanisms. Here we describe a robust and relatively high-throughput flow cytometry assay to quantify phagocytosis of by human neutrophils. We show that effective phagocytic uptake of is greatly enhanced by opsonization, i.e. the tagging of microbial surfaces with plasma-derived host proteins like antibodies and complement. Our rapid assay to monitor phagocytosis can be used to study neutrophil deficiencies and bacterial evasion, but also provides a powerful tool to assess the opsonic capacity of antibodies, either in the context of natural immune responses or immune therapies.
中性粒细胞在人体对感染的免疫反应中发挥着关键作用。这些专业的吞噬细胞迅速迁移到感染部位，吞噬细菌并通过专门的细胞内杀伤机制将其摧毁。在这里，我们描述了一种强大且相对高通量的流式细胞术检测法，用于定量检测人中性粒细胞对 的吞噬作用。我们发现，通过调理作用（即通过血浆来源的宿主蛋白如抗体和补体对微生物表面进行标记），可大大增强对 的有效吞噬作用。我们的快速检测法可用于研究中性粒细胞缺陷和细菌逃避，同时也为评估抗体的调理能力提供了有力工具，无论是在天然免疫反应还是免疫治疗的背景下。
