[Reactivity to Staphylococcus aureus peptidoglycan in a neutrophil phagocytosis system].

The mechanisms of the formation of reactivity in the system "S. aureus peptidoglycan--serum--neutrophils" were studied. The direct interaction of peptidoglycans with neutrophils isolated from human blood was not accompanied by their stimulation, evaluated by an increase in the reduction of nitro blue tetrazolium. Antibodies to peptidoglycan determined the final result of the opsonic activity of normal serum: the IgG fraction isolated from commercial immunoglobulin was active at a concentration of 1 mg/ml. The activity of complement was considerably lower: it was manifested after the removal of antibodies and in cooperation with suboptimal dosage. The IgG-independent activation of complement was equally blocked by 10 mM EDTA and Mg-EGTA. Residual activity (after the removal of antibodies an the blocking of complement) constituted an insignificant proportion of the total opsonic reserve of the serum. The specific features of the stimulating activity of peptidoglycans obtained from different S. aureus strains were observed.