Boiarinova L V, Salina E V, Maianskaia I V, Maianskiĭ A N
Zh Mikrobiol Epidemiol Immunobiol. 1983 Dec(12):49-53.
The mechanisms of the formation of reactivity in the system "S. aureus peptidoglycan--serum--neutrophils" were studied. The direct interaction of peptidoglycans with neutrophils isolated from human blood was not accompanied by their stimulation, evaluated by an increase in the reduction of nitro blue tetrazolium. Antibodies to peptidoglycan determined the final result of the opsonic activity of normal serum: the IgG fraction isolated from commercial immunoglobulin was active at a concentration of 1 mg/ml. The activity of complement was considerably lower: it was manifested after the removal of antibodies and in cooperation with suboptimal dosage. The IgG-independent activation of complement was equally blocked by 10 mM EDTA and Mg-EGTA. Residual activity (after the removal of antibodies an the blocking of complement) constituted an insignificant proportion of the total opsonic reserve of the serum. The specific features of the stimulating activity of peptidoglycans obtained from different S. aureus strains were observed.
研究了“金黄色葡萄球菌肽聚糖 - 血清 - 中性粒细胞”系统中反应性形成的机制。肽聚糖与人血中分离出的中性粒细胞的直接相互作用，并未伴随着通过硝基蓝四氮唑还原增加所评估的细胞刺激。肽聚糖抗体决定了正常血清调理活性的最终结果：从商业免疫球蛋白中分离出的IgG组分在浓度为1mg/ml时具有活性。补体的活性要低得多：在去除抗体后并与次优剂量协同作用时才表现出来。IgG非依赖性补体激活同样被10mM EDTA和Mg - EGTA阻断。残余活性（去除抗体并阻断补体后）在血清总调理储备中占比微不足道。观察到了从不同金黄色葡萄球菌菌株获得的肽聚糖刺激活性的特异性特征。
