Hao Xiaoyan, Ma Cong, Xiang Tianyuan, Ou Lei, Zeng Qiang
Health Management Institute, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, China.
Front Genet. 2021 Feb 18;12:568398. doi: 10.3389/fgene.2021.568398. eCollection 2021.
To investigate the associations among the methylene tetrahydrofolate reductase rs1801133 gene variant, food groups, and the risk of non-alcoholic fatty liver disease in the Chinese population.
A study of gene polymorphism was conducted using the polymerase chain reaction method. A total of 4,049 adults participated in the study, and all underwent physical examination and genotyping. Participants filled out a dietary questionnaire to enable us to assess the frequency and quantity of food consumption.
The important variables identified as risk factors of non-alcoholic fatty liver disease were age, smoking, sex, body mass index, hyperlipidemia, diabetes, and methylene tetrahydrofolate reductase genotype (T - allele carriers). The homocysteine content was higher in the non-alcoholic fatty liver disease group than in the control group, and was higher in the T- allele than C- allele carriers. The homocysteine content was the highest in the T- allele carriers. Additionally, certain food groups such as milk and beans were associated with a lower risk of non-alcoholic fatty liver disease. Food groups such as meat, were associated with a higher risk of non-alcoholic fatty liver disease. Fresh fruit and vegetables, salted and smoked foods, desserts, cereals, fish, and eggs were not associated with the risk of non-alcoholic fatty liver disease. However, the influence of salted and smoked foods on non-alcoholic fatty liver disease was different in the C-allele and T-allele carriers of methylene tetrahydrofolate reductase (CT + TT vs. CC, OR = 1.196, = 0.041 for 1-4 times food per week, OR = 1.580, = 0.004 for 5-7 times per week). Similarly, salted and smoked foods were also a risk factor for the development of non-alcoholic steatohepatitis in patients with non-alcoholic fatty liver disease.
This study found that the T-allele of the variant of methylene tetrahydrofolate reductase was a risk factor for non-alcoholic fatty liver disease among Chinese people. These results can likely aid the development of novel approaches for managing non-alcoholic fatty liver disease risk.
研究亚甲基四氢叶酸还原酶rs1801133基因变异、食物种类与中国人群非酒精性脂肪性肝病风险之间的关联。
采用聚合酶链反应方法进行基因多态性研究。共有4049名成年人参与该研究，所有人均接受了体格检查和基因分型。参与者填写了饮食问卷，以便我们评估食物消费的频率和数量。
被确定为非酒精性脂肪性肝病危险因素的重要变量包括年龄、吸烟、性别、体重指数、高脂血症、糖尿病和亚甲基四氢叶酸还原酶基因型（T等位基因携带者）。非酒精性脂肪性肝病组的同型半胱氨酸含量高于对照组，T等位基因携带者的同型半胱氨酸含量高于C等位基因携带者。T等位基因携带者的同型半胱氨酸含量最高。此外，某些食物种类，如牛奶和豆类，与较低的非酒精性脂肪性肝病风险相关。肉类等食物种类与较高的非酒精性脂肪性肝病风险相关。新鲜水果和蔬菜、腌制和烟熏食品、甜点、谷物、鱼类和蛋类与非酒精性脂肪性肝病风险无关。然而，腌制和烟熏食品对非酒精性脂肪性肝病的影响在亚甲基四氢叶酸还原酶的C等位基因和T等位基因携带者中有所不同（CT+TT与CC相比，每周食用1 - 4次食物时，OR = 1.196，P = 0.041；每周食用5 - 7次时，OR = 1.580，P = 0.004）。同样，腌制和烟熏食品也是非酒精性脂肪性肝病患者发生非酒精性脂肪性肝炎的危险因素。
本研究发现，亚甲基四氢叶酸还原酶变异体的T等位基因是中国人非酒精性脂肪性肝病的危险因素。这些结果可能有助于开发管理非酒精性脂肪性肝病风险的新方法。
