Discovery of a Novel Lineage ST 1870 Endophytically Isolated from Medicinal Which Shows Potent Antileishmanial and Antimicrobial Effects.

In this study, we report the isolation and identification of an endophytic strain of (COPS strain) associated with roots. plants are rich sources of promising microbiomes, of which the literature reports several pharmacological effects, such as trypanocidal, antinociceptive, anesthetic, anxiolytics, and anticonvulsant activities. COPS belongs to a new sequence type (ST 1870) and harbors a genome estimated in 8.3 Mbp which exhibits the aminoglycosides and beta-lactams resistance genes (3')-IIa and , respectively. Analysis performed using MLST, average nucleotide identity, and digital DNA-DNA hybridization support its species-level identification and reveals its novel housekeeping genes alleles B, A, and C. The root endophyte COPS drew our attention from a group of 14 bacterial isolates during the primary screening for being potentially active against ATCC 29213, ATCC 29212, ATCC 9341, ATCC 25922, and ATCC 10231 and exhibited the broad-spectrum activity against phytopathogenic fungi. In addition, COPS strain showed production of protease, lipase, and esterase in solid media, and its natural product extract showed potent inhibition against fungal plant pathogens, such as , whose antagonism index (89.32%) exceeded the positive control (74.17%), whereas and showed high percentages of inhibition (85.53% and 82.69%, respectively). COPS crude extract also significantly inhibited ATCC 35984, ATCC 700221 (MIC values of 32 g/mL for both), ATCC 29212 (64 g/mL), and ATCC 25923 (128 g/mL). We observed moderate antagonistic activity against ATCC 19606 and ATCC 25922 (both at 512 g/mL), as well as potent cytotoxic effects on and promastigote forms with 78.25% and 57.30% inhibition. In conclusion, this study presents for the first time the isolation of an endophytic strain associated with and enough evidence that these plants may be considered a rich source of microbes for the fight against neglected diseases.