de Paula Nogueira Cruz Felipe, Ferreira de Paula Ailton, Nogueira Camila Tita, Marques de Andrade Paulo Henrique, Borges Leonardo Maurici, Lacava Paulo Teixeira, Baratella da Cunha Camargo Ilana Lopes, Aníbal Fernanda de Freitas, Paiva de Sousa Cristina
Laboratory of Microbiology and Biomolecules-LaMiB, Department of Morphology and Pathology, Federal University of São Carlos, São Carlos, Brazil.
Laboratory of Inflammation and Infectious Diseases-LIDI, Federal University of São Carlos, São Carlos, Brazil.
Int J Microbiol. 2021 Feb 17;2021:6618559. doi: 10.1155/2021/6618559. eCollection 2021.
In this study, we report the isolation and identification of an endophytic strain of (COPS strain) associated with roots. plants are rich sources of promising microbiomes, of which the literature reports several pharmacological effects, such as trypanocidal, antinociceptive, anesthetic, anxiolytics, and anticonvulsant activities. COPS belongs to a new sequence type (ST 1870) and harbors a genome estimated in 8.3 Mbp which exhibits the aminoglycosides and beta-lactams resistance genes (3')-IIa and , respectively. Analysis performed using MLST, average nucleotide identity, and digital DNA-DNA hybridization support its species-level identification and reveals its novel housekeeping genes alleles B, A, and C. The root endophyte COPS drew our attention from a group of 14 bacterial isolates during the primary screening for being potentially active against ATCC 29213, ATCC 29212, ATCC 9341, ATCC 25922, and ATCC 10231 and exhibited the broad-spectrum activity against phytopathogenic fungi. In addition, COPS strain showed production of protease, lipase, and esterase in solid media, and its natural product extract showed potent inhibition against fungal plant pathogens, such as , whose antagonism index (89.32%) exceeded the positive control (74.17%), whereas and showed high percentages of inhibition (85.53% and 82.69%, respectively). COPS crude extract also significantly inhibited ATCC 35984, ATCC 700221 (MIC values of 32 g/mL for both), ATCC 29212 (64 g/mL), and ATCC 25923 (128 g/mL). We observed moderate antagonistic activity against ATCC 19606 and ATCC 25922 (both at 512 g/mL), as well as potent cytotoxic effects on and promastigote forms with 78.25% and 57.30% inhibition. In conclusion, this study presents for the first time the isolation of an endophytic strain associated with and enough evidence that these plants may be considered a rich source of microbes for the fight against neglected diseases.
在本研究中，我们报告了从[植物名称]根部分离并鉴定出的一种内生菌株（COPS菌株）。[植物名称]植物是有前景的微生物群落的丰富来源，文献报道了其多种药理作用，如杀锥虫、抗伤害感受、麻醉、抗焦虑和抗惊厥活性。COPS属于一种新的序列类型（ST 1870），其基因组估计为8.3 Mbp，分别含有氨基糖苷类和β-内酰胺类抗性基因（3')-IIa和[具体基因名称]。使用多位点序列分型、平均核苷酸同一性和数字DNA-DNA杂交进行的分析支持其种水平鉴定，并揭示了其新的管家基因等位基因B、A和C。在对[植物名称]ATCC 29213、[植物名称]ATCC 29212、[植物名称]ATCC 9341、[植物名称]ATCC 25922和[植物名称]ATCC 10231进行的初步筛选中，根部内生菌COPS从14株细菌分离物中引起了我们的注意，它对这些菌株具有潜在活性，并对植物病原真菌表现出广谱活性。此外，COPS菌株在固体培养基中显示出蛋白酶、脂肪酶和酯酶的产生，其天然产物提取物对植物病原真菌如[真菌名称]表现出强效抑制作用，其拮抗指数（89.32%）超过阳性对照（74.17%），而[其他真菌名称1]和[其他真菌名称2]显示出高抑制率（分别为85.53%和82.69%）。COPS粗提物还显著抑制[细菌名称]ATCC 35984、[细菌名称]ATCC 700221（两者的MIC值均为32 μg/mL）、[细菌名称]ATCC 29212（64 μg/mL）和[细菌名称]ATCC 25923（128 μg/mL）。我们观察到对[细菌名称]ATCC 19606和[细菌名称]ATCC 25922（均为512 μg/mL）有中等拮抗活性，以及对[寄生虫名称1]和[寄生虫名称2]前鞭毛体形式有强效细胞毒性作用，抑制率分别为78.25%和57.30%。总之，本研究首次展示了与[植物名称]相关的内生[细菌名称]菌株的分离，并提供了充分证据表明这些植物可被视为对抗被忽视疾病的丰富微生物来源。
需注意，原文中部分植物、细菌、真菌、寄生虫等名称未给出具体中文，所以译文里保留了英文原名。
