β-谷甾醇介导的银纳米颗粒对人结肠癌HT-29细胞具有细胞毒性。
β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells.
作者信息
Shathviha Palaniappan Chithambara, Ezhilarasan Devaraj, Rajeshkumar Shanmugam, Selvaraj Jayaraman
机构信息
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India.
Department of Biochemistry, Saveetha Dental College (SDC), Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.
出版信息
Avicenna J Med Biotechnol. 2021 Jan-Mar;13(1):42-46. doi: 10.18502/ajmb.v13i1.4577.
BACKGROUND
Silver nanoparticles (AgNP) are commonly used metallic nanoparticles in health care systems. Colon cancer incidence is increasing worldwide. In this study, AgNP was synthesized using β-sitosterol and its cytotoxic potential was evaluated in human colon cancer (HT-29) cells.
METHODS
Characterization of AgNP was analyzed by TEM and spectrophotometry analysis. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ) of AgNPs and cytotoxicity was evaluated by MTT assay. The apoptosis was analyzed by the flow cytometry. The expression of p53 protein was analyzed by western blotting.
RESULTS
β-sitosterol mediated AgNP are spherical in shape and induced concentration-dependent cytotoxicity in HT-29 cells. AgNP caused apoptosis related morphological changes as evidenced by annexin positive staining. AgNP treatments also induced the p53 expression in HT-29 cells.
CONCLUSION
Our present result suggests that β-sitosterol mediated AgNP induce apoptosis in colon cancer cells and this finding may pave the way for further experimental analysis .
背景
银纳米颗粒（AgNP）是医疗保健系统中常用的金属纳米颗粒。全球范围内结肠癌的发病率正在上升。在本研究中，使用β-谷甾醇合成了AgNP，并在人结肠癌细胞（HT-29）中评估了其细胞毒性潜力。
方法
通过透射电子显微镜（TEM）和分光光度法分析对AgNP进行表征。用不同浓度（2、4、6、8和10）的AgNP处理HT-29细胞，并通过MTT法评估细胞毒性。通过流式细胞术分析细胞凋亡。通过蛋白质印迹法分析p53蛋白的表达。
结果
β-谷甾醇介导的AgNP呈球形，并在HT-29细胞中诱导浓度依赖性细胞毒性。膜联蛋白阳性染色证明AgNP引起了与细胞凋亡相关的形态学变化。AgNP处理还诱导了HT-29细胞中p53的表达。
结论
我们目前的结果表明，β-谷甾醇介导的AgNP可诱导结肠癌细胞凋亡，这一发现可能为进一步的实验分析铺平道路。
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