长链非编码RNA UCA1与子宫内膜基质细胞的自噬和凋亡相关。
Long Noncoding RNA UCA1 Is Related to Autophagy and Apoptosis in Endometrial Stromal Cells.
作者信息
Jiang Lili, Wan Yahui, Feng Ziyi, Liu Da, Ouyang Ling, Li Yan, Liu Kuiran
机构信息
Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, China.
College of Clinical Medicine Science, China Medical University, Shenyang, China.
出版信息
Front Oncol. 2021 Feb 18;10:618472. doi: 10.3389/fonc.2020.618472. eCollection 2020.
RESEARCH QUESTION
The expression of the long noncoding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) in embryonic tissues is higher than that in most cancer tissues, such as bladder cancer, indicating that RNA is a carcinoembryonic antigen. However, there are no published reports on the role of UCA1 in endometriosis (EMS). Therefore, to address this gap in knowledge, we assessed the potential role of lncRNA UCA1 in the pathogenesis and progression of EMS.
DESIGN
To verify the expression of UCA1 in EMS, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used. RNA interference (siRNA) was used to study the biological function of UCA1 in EMS .
RESULTS
qRT-PCR analysis showed that the expression of lncRNA UCA1 in EMS was increased (P<0.01). Knockdown of UCA1 significantly inhibited the proliferation of endometrial stromal cells (ESCs) and induced autophagy and apoptosis.
CONCLUSION
UCA1 is highly expressed in EMS and promotes the proliferation of ESCs but suppresses autophagy and apoptosis. In EMS, UCA1 may be a prognostic marker and therapeutic target.
研究问题
长链非编码RNA（lncRNA）尿路上皮癌相关1（UCA1）在胚胎组织中的表达高于大多数癌症组织，如膀胱癌，这表明该RNA是一种癌胚抗原。然而，关于UCA1在子宫内膜异位症（EMS）中的作用尚无发表的报告。因此，为填补这一知识空白，我们评估了lncRNA UCA1在EMS发病机制和进展中的潜在作用。
设计
为验证UCA1在EMS中的表达，采用了定量逆转录聚合酶链反应（qRT-PCR）。使用RNA干扰（siRNA）研究UCA1在EMS中的生物学功能。
结果
qRT-PCR分析表明，lncRNA UCA1在EMS中的表达增加（P<0.01）。敲低UCA1可显著抑制子宫内膜基质细胞（ESC）的增殖，并诱导自噬和凋亡。
结论
UCA1在EMS中高表达，促进ESC增殖，但抑制自噬和凋亡。在EMS中，UCA1可能是一种预后标志物和治疗靶点。
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