Hernandes Camila, Silveira Paola, Rodrigues Sereia Aline Fernanda, Christoff Ana Paula, Mendes Helen, Valter de Oliveira Luiz Felipe, Podgaec Sergio
Hospital Israelita Albert Einstein, Av. Albert Einstein 627, Morumbi, São Paulo 05651-901, Brazil.
BiomeHub, Av. Luiz Boiteux Piazza, 1302, Canasvieiras, Florianópolis 88056-000, Brazil.
Diagnostics (Basel). 2020 Mar 17;10(3):163. doi: 10.3390/diagnostics10030163.
This work aimed to identify and compare the bacterial patterns present in endometriotic lesions, eutopic endometrium and vaginal fluid from endometriosis patients with those found in the vaginal fluid and eutopic endometrium of control patients. Vaginal fluid, eutopic endometrium and endometriotic lesions were collected. DNA was extracted and the samples were analyzed to identify microbiome by high-throughput DNA sequencing of the 16S rRNA marker gene. Amplicon sequencing from vaginal fluid, eutopic endometrium and endometriotic lesion resulted in similar profiles of microorganisms, composed most abundantly by the genus and . No significant differences were found in the diversity analysis of microbiome profiles between control and endometriotic patients; however deep endometriotic lesions seems to present different bacterial composition, less predominant of and with more abundant and
这项研究旨在识别并比较子宫内膜异位症患者的异位内膜病变、在位内膜及阴道分泌物中的细菌模式，以及对照患者阴道分泌物和在位内膜中的细菌模式。收集了阴道分泌物、在位内膜及异位内膜病变组织。提取DNA，并通过对16S rRNA标记基因进行高通量DNA测序来分析样本，以鉴定微生物群。对阴道分泌物、在位内膜及异位内膜病变组织进行扩增子测序，得到了相似的微生物谱，其中最主要的是属和属。在对照患者和子宫内膜异位症患者的微生物谱多样性分析中未发现显著差异；然而，深部异位内膜病变似乎呈现出不同的细菌组成，属的占比更少，而属和属更为丰富。
