Department of Neurology, The Third Affiliated Hospital of SUN Yat-sen University, 600 Tianhe Road, Guangzhou, Guangdong 510630, China.
State Key Laboratory for Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
STAR Protoc. 2021 Feb 27;2(1):100374. doi: 10.1016/j.xpro.2021.100374. eCollection 2021 Mar 19.
Adult neurogenesis, a process of generating newborn neurons from adult neural stem cells, is required for brain homeostasis, cognition, and affective behaviors. Deciphering the molecular mechanisms underlying adult neurogenesis will provide valuable insights into the functional integrity of the adult brain and the etiology of neurological disorders. Here, we present an optimized protocol combining stereotactic injection of retrovirus expressing red fluorescent protein to label newborn neurons and implantation of a mini-osmotic pump to investigate newborn neuron development in adult mouse hippocampus. For complete details on the use and execution of this protocol, please refer to Tang et al. (2019).
成人神经发生，即从成体神经干细胞产生新生神经元的过程，是大脑内稳态、认知和情感行为所必需的。解析成人神经发生的分子机制将为理解成年大脑的功能完整性和神经障碍的病因提供有价值的线索。在这里，我们提出了一种优化的方案，该方案结合了立体定位注射表达红色荧光蛋白的逆转录病毒以标记新生神经元和植入迷你渗透泵来研究成年小鼠海马中的新生神经元发育。有关该方案使用和执行的完整详细信息，请参考 Tang 等人（2019 年）。
