Bozhkova V P, Sharova L V, Petriaevskaia V B, Kozlov D A, Khrust Iu R
Ontogenez. 1988 Jan-Feb;19(1):73-81.
The unfertilized eggs of sand-dollars (Scaphechinus mirabilis) were treated with local anesthetics (novocaine, lidocaine, trimecaine) or phorbol ester for 10 min. The treatment with the local anesthetics increased pH of the cytoplasm in the unfertilized eggs (pHc) and changed the value of the pHc increment in response to fertilization, as compared with the control eggs. In the treated embryos, the onset of immigration of the primary mesenchyme cells was delayed but the hatching was accelerated. The phorbol ester treatment rapidly accelerated development from gastrulation on and practically did not affect the preceding stages.
用局部麻醉剂（奴佛卡因、利多卡因、三甲卡因）或佛波酯处理沙钱（奇异刻肋海胆）的未受精卵10分钟。与对照卵相比，局部麻醉剂处理增加了未受精卵细胞质的pH值（pHc），并改变了受精时pHc增量的值。在经处理的胚胎中，初级间充质细胞迁移的起始延迟，但孵化加速。佛波酯处理从原肠胚形成期开始迅速加速发育，而实际上对之前的阶段没有影响。
