Bozhkova V P, Petriaevskaia V B, Chaĭlakhian L M, Khrust Iu R
Ontogenez. 1990 Mar-Apr;21(2):160-6.
Phorbol-12-myristate-13-acetate (PMA) treatment (9-170 nM) of S. mirabilis embryos from the fertilization to the IV cleavage division resulted in morphogenesis disturbances, such as decrease of the intercellular contacts' extent of blastomeres during the first cleavage divisions, inhibition of micromere formation, and formation of multiple blastulae. After washing from PMA embryos remained viable up to the pluteus stage. Morphogenetic disturbances were not accompanied by changes of intracellular pH or mechanical properties of blastomeres' surface during cleavage. Protein kinase C is suggested to control adhesion of the cell surface and intracellular nucleus movements.
用佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA，9-170 nM）处理奇异海胆从受精到第四次卵裂期的胚胎，会导致形态发生紊乱，例如在第一次卵裂期卵裂球间细胞接触范围减小、微裂球形成受到抑制以及形成多个囊胚。从PMA中洗脱后，胚胎在发育至长腕幼虫阶段前仍保持存活。形态发生紊乱在卵裂过程中并未伴随细胞内pH值的变化或卵裂球表面力学性质的改变。推测蛋白激酶C可控制细胞表面的黏附以及细胞核在细胞内的移动。
