Department of Orthopaedic Surgery, Orthopaedic Trauma Institute, University of California.
Department of Orthopaedic Surgery, Orthopaedic Trauma Institute, University of California;
J Vis Exp. 2021 Feb 18(168). doi: 10.3791/62183.
The avian embryo has been used as a model system for more than a century and has led to fundamental understanding of vertebrate development. One of the strengths of this model system is that the effect of, and interaction among, tissues can be directly assessed in chimeric embryos. We have previously shown that signals from the forebrain contribute to facial morphogenesis by regulating the shape of the expression domain of Sonic hedgehog (SHH) in the Frontonasal Ectodermal Zone (FEZ). In this article, the method of generating the forebrain chimeras and provide illustrations of the outcomes of these experiments is described.
鸟类胚胎作为一个模型系统已经被使用了一个多世纪，并对脊椎动物的发育有了基本的了解。这个模型系统的优势之一是可以在嵌合体胚胎中直接评估组织的作用和相互作用。我们之前已经表明，来自前脑的信号通过调节 Sonic hedgehog（SHH）在额鼻外胚层区（FEZ）中的表达域的形状来影响面部形态发生。在本文中，描述了生成前脑嵌合体的方法，并提供了这些实验结果的说明。
