桃儿七醇提物的抑菌活性及其对人源抗生素耐药菌的抑制作用。

Pak J Biol Sci. 2024 Mar;27(3):119-124. doi: 10.3923/pjbs.2024.119.124.

Background and Objective: A new strain of cannabis, Cannabis sativa L. Tanao Si Kan Dang RD1, has been approved and registered by the Rajamangala University of Technology Isan, Thailand. The C. sativa is acknowledged for its medicinal properties which demonstrated various therapeutic properties, such as anti-cancer and antibacterial activities. This study aimed to investigate the antibacterial activity of ethanolic extracts from the stems and leaves of the Tanao Si Kan Dang RD1 strain against seven antibiotic-resistant bacteria. Materials and Methods: The primary antibacterial activity of ethanolic Tanao Si Kan Dang RD1 extracts were determined using the disc diffusion method, while the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined using the broth microdilution method. Results: The largest inhibition zone, measuring 12 mm, was observed in leaf extracts against Pseudomonas aeruginosa 101. The lowest MIC, at 0.78 mg/mL, was obtained from stem extracts against Stenotrophomonas maltophilia. The lowest MBCs, at 12.5 mg/mL, were observed in leaf extracts against Enterococcus faecalis, Acinetobacter baumannii, multidrug-resistant Klebsiella pneumoniae, Stenotrophomonas maltophilia and Pseudomonas aeruginosa 101 and stem extracts against Acinetobacter baumannii, multidrug-resistant Klebsiella pneumoniae, Stenotrophomonas maltophilia and Pseudomonas aeruginosa 101. Conclusion: This study presents a novel finding regarding the antibacterial activity of ethanolic extracts from the leaves and stems of Tanao Si Kan Dang RD1 against antibiotic-resistant bacteria. The potential application of these cannabis plant extracts in the development of antibiotics capable of combating antibiotic-resistant pathogenic bacteria represents a promising strategy to address a significant global health concern.

背景与目的

泰国拉差蒙坤理工大学已批准并注册了一种新的大麻品种，即Cannabis sativaL. Tanao Si Kan Dang RD1。该C. sativa因其药用特性而备受认可，具有多种治疗特性，如抗癌和抗菌活性。本研究旨在探讨 Tanao Si Kan Dang RD1 菌株茎叶乙醇提取物对七种抗生素耐药菌的抗菌活性。材料与方法：采用纸片扩散法测定 Tanao Si Kan Dang RD1 乙醇提取物的初步抗菌活性，采用微量肉汤稀释法测定最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。结果：叶片提取物对铜绿假单胞菌101 的抑菌圈最大，为 12mm。茎提取物对嗜麦芽寡养单胞菌的 MIC 最低，为 0.78mg/ml。叶片提取物对粪肠球菌、鲍曼不动杆菌、多重耐药肺炎克雷伯菌、嗜麦芽寡养单胞菌和铜绿假单胞菌101 的 MBCs 最低，为 12.5mg/ml；茎提取物对鲍曼不动杆菌、多重耐药肺炎克雷伯菌、嗜麦芽寡养单胞菌和铜绿假单胞菌101 的 MBCs 最低，为 12.5mg/ml。结论：本研究首次发现 Tanao Si Kan Dang RD1 茎叶乙醇提取物对抗生素耐药菌具有抗菌活性。这些大麻植物提取物在开发能够对抗抗生素耐药性病原菌的抗生素方面具有潜在应用，这是解决重大全球健康问题的一种有前途的策略。