Khafizov K F, Petrov V V, Krasovitov K V, Zolkina M V, Akimkin V G
FSBI Central Research Institute for Epidemiology of the Federal Service for Surveillance of Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor).
Vopr Virusol. 2021 Mar 7;66(1):17-28. doi: 10.36233/0507-4088-42.
This review presents the basic principles of application of the loop-mediated isothermal amplification (LAMP) reaction for the rapid diagnosis of coronavirus infection caused by SARS-CoV-2. The basic technical details of the method, and the most popular approaches of specific and non-specific detection of amplification products are briefly described. We also discuss the first published works on the use of the method for the detection of the nucleic acid of the SARS-CoV-2 virus, including those being developed in the Russian Federation. For commercially available and published LAMP-based assays, the main analytical characteristics of the tests are listed, which are often comparable to those based on the method of reverse transcription polymerase chain reaction (RT-PCR), and in some cases are even superior. The advantages and limitations of this promising methodology in comparison to other methods of molecular diagnostics, primarily RT-PCR, are discussed, as well as the prospects for the development of technology for the detection of other infectious agents.
本综述介绍了环介导等温扩增（LAMP）反应在快速诊断由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的冠状病毒感染中的应用基本原则。简要描述了该方法的基本技术细节，以及扩增产物特异性和非特异性检测的最常用方法。我们还讨论了关于使用该方法检测SARS-CoV-2病毒核酸的首批发表作品，包括俄罗斯联邦正在研发的作品。对于市售的和已发表的基于LAMP的检测方法，列出了检测的主要分析特征，这些特征通常与基于逆转录聚合酶链反应（RT-PCR）方法的特征相当，在某些情况下甚至更优。讨论了这种有前景的方法与其他分子诊断方法（主要是RT-PCR）相比的优点和局限性，以及检测其他传染病原体技术的发展前景。
