Department of Pharmaceutical Analysis, China Pharmaceutical University, No. 24, Tongjiaxiang, Nanjing 210009, China.
Anal Methods. 2021 Mar 21;13(11):1412-1421. doi: 10.1039/d0ay02025g. Epub 2021 Mar 8.
Core-satellite-structured magnetic nanosorbents (MNs) used for the selective extraction of macrolide antibiotics (MACs) were prepared in this study. The MNs (core-satellite polydopamine-coated FeO nanoparticles-hollow porous molecularly imprinted polymer) consisted of polydopamine-coated FeO nanoparticles (FeO@PDA) "core" linked to numerous hollow porous molecularly imprinted polymer (HPMIP) "satellites" with bridging amine functional groups. It is worth mentioning that HPMIPs act as "anchors" for selectively capturing target molecules. Polymers were characterized using TEM, SEM, FT-IR, VSM, and TGA and applied as magnetic dispersive solid-phase extraction (MDSPE) sorbents for the enrichment of trace MACs from a complex food matrix prior to quantification by HPLC-MS/MS. Nanocomposites revealed outstanding magnetic properties (36.1 emu g), a high adsorption capacity (103.6 μmol g), selectivity (IF = 3.2), and fast kinetic binding (20 min) for MACs. The multiple advantages of the novel core-satellite-structured magnetic molecularly imprinted nanosorbents were confirmed, which makes us believe that the preparation method of the core-satellite MNs can be applied to other fields involving molecular imprinting technology.
本研究制备了用于选择性提取大环内酯类抗生素 (MACs) 的核-卫星结构磁性纳米吸附剂 (MNs)。MNs (核-卫星聚多巴胺包覆 FeO 纳米粒子-中空多孔分子印迹聚合物) 由聚多巴胺包覆的 FeO 纳米粒子 (FeO@PDA) “核”与许多带有桥连胺官能团的中空多孔分子印迹聚合物 (HPMIP) “卫星”相连组成。值得一提的是，HPMIPs 充当选择性捕获目标分子的“锚点”。使用 TEM、SEM、FT-IR、VSM 和 TGA 对聚合物进行了表征，并将其用作磁性分散固相萃取 (MDSPE) 吸附剂，用于从复杂食品基质中富集痕量 MACs，然后通过 HPLC-MS/MS 进行定量分析。纳米复合材料表现出出色的磁性 (36.1 emu g)、高吸附容量 (103.6 μmol g)、选择性 (IF = 3.2) 和快速动力学结合 (20 min) ，可用于 MACs。新型核-卫星结构磁性分子印迹纳米吸附剂的多种优势得到了证实，这使我们相信核-卫星 MNs 的制备方法可以应用于涉及分子印迹技术的其他领域。
