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热液和水热时间模型描述了油菜种子对老化的发芽反应。

Halothermal and hydrothermal time models describe germination responses of canola seeds to ageing.

机构信息

Genetics and Agricultural Biotechnology Institute of Tabarestan & Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

Department of Agroecology, Environmental Sciences Research Institute, Shahid Beheshti University, Tehran, Iran.

出版信息

Plant Biol (Stuttg). 2021 Jul;23(4):621-629. doi: 10.1111/plb.13251. Epub 2021 Apr 23.

Abstract

Hydrothermal time (HTT) and halothermal time (HaloTT) models were used to quantitatively characterize the combined effects of temperature (T), water potential (ψ) and NaCl concentration on seed germination of canola after different accelerated ageing periods (AAP) at 42 °C and 100% humidity. Seed germination time courses were observed and electrical conductivities of seed leachates were measured in all experiments. The cardinal temperatures estimated by both models were 4.6, 28 and 35 °C for the base (T ), optimum (T ) and ceiling (T ) temperatures in water (0 MPa), respectively, when the seeds were not aged. The T increased with AAP, while the T decreased and the T remained constant at all AAP. Below T , the median base water potentials (ψ (50)) were -1.07, -0.73, -0.48 and -0.39 MPa for the AAP levels of 0, 24, 36 and 48 h, respectively. These values were more negative when germination occurred in salt solutions (the base NaCl concentrations converted to ψ were -1.25, -0.81, -0.51 and -0.41 MPa, respectively), due to uptake of salt ions. Thresholds became more positive above T and reached zero at T in all AAP. Seed osmotic adjustment capacity declined linearly with increasing AAP while the conductivity of seed leachates increased in association with the loss of seed vigour. Significant correlations between conductivity results and the parameters of HTT and HaloTT models suggest that they could be considered as effective descriptors of canola seed vigour.

摘要

水热时间 (HTT) 和热盐时间 (HaloTT) 模型被用来定量描述温度 (T)、水势 (ψ) 和 NaCl 浓度对经过不同加速老化期 (AAP) 在 42°C 和 100%湿度下油菜种子萌发的综合影响。在所有实验中观察了种子萌发时间过程,并测量了种子浸出液的电导率。当种子未老化时,两种模型估计的特征温度分别为 4.6、28 和 35°C,分别为水(0 MPa)中的基础温度 (Tbase)、最佳温度 (Topt) 和上限温度 (Tceiling)。T 随 AAP 而增加,而 T 下降,T 在所有 AAP 下保持不变。低于 T 时,老化水平为 0、24、36 和 48 h 的种子的中位基础水势 (ψ (50)) 分别为-1.07、-0.73、-0.48 和-0.39 MPa。当在盐溶液中发生萌发时,这些值更负(将基础 NaCl 浓度转换为 ψ 的值分别为-1.25、-0.81、-0.51 和-0.41 MPa),因为盐离子的吸收。在所有 AAP 中,阈值在 T 以上变得更正,并在 T 处变为零。种子渗透调节能力随 AAP 的增加而线性下降,而种子浸出液的电导率随着种子活力的丧失而增加。电导率结果与 HTT 和 HaloTT 模型参数之间的显著相关性表明,它们可以被认为是油菜种子活力的有效描述符。

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