时间分辨和综合表面糖蛋白分析揭示了单核细胞和巨噬细胞对细菌感染的不同反应。
Time-Resolved and Comprehensive Analysis of Surface Glycoproteins Reveals Distinct Responses of Monocytes and Macrophages to Bacterial Infection.
机构信息
School of Chemistry and Biochemistry, and the, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, 30332, USA.
出版信息
Angew Chem Int Ed Engl. 2021 May 10;60(20):11494-11503. doi: 10.1002/anie.202102692. Epub 2021 Apr 6.
Abstract
Glycoproteins on the surface of immune cells play extremely important roles in response to pathogens. Yet, a systematic and time-resolved investigation of surface glycoproteins during the immune response remains to be explored. Integrating selective enrichment of surface glycoproteins with multiplexed proteomics, we globally and site-specifically quantified the dynamics of surface glycoproteins on THP-1 monocytes and macrophages in response to bacterial infection and during the monocyte-to-macrophage differentiation. The time-resolved analysis reveals transient changes and differential remodeling of surface glycoproteins on both cell types, and potential upstream regulators and downstream effects of the regulated glycoproteins. Besides, we identified novel surface glycoproteins participating in the immune response such as APMAP, and site-specific changes of glycoproteins. This study provides unprecedented information to deepen our understanding of glycoproteins and cellular activities.
摘要
免疫细胞表面的糖蛋白在对病原体的反应中起着极其重要的作用。然而，在免疫反应过程中，对表面糖蛋白进行系统的和时间分辨的研究仍有待探索。本研究将表面糖蛋白的选择性富集与多重蛋白质组学相结合，全面、特异性地定量分析了 THP-1 单核细胞和巨噬细胞在细菌感染和单核细胞向巨噬细胞分化过程中表面糖蛋白的动态变化。时间分辨分析揭示了两种细胞类型表面糖蛋白的瞬时变化和差异重塑，以及受调控糖蛋白的潜在上游调节剂和下游效应。此外，我们还鉴定了参与免疫反应的新型表面糖蛋白，如 APMAP，以及糖蛋白的特异性变化。这项研究提供了前所未有的信息，加深了我们对糖蛋白和细胞活动的理解。
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