BBSRC Midlands Integrative Biosciences Training Partnership, University of Warwick, Coventry, CV4 7AL, UK.
School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
Environ Microbiol. 2021 Sep;23(9):5069-5086. doi: 10.1111/1462-2920.15451. Epub 2021 Mar 13.
The Burkholderia cepacia complex is a group of Burkholderia species that are opportunistic pathogens causing high mortality rates in patients with cystic fibrosis. An environmental stress often encountered by these soil-dwelling and pathogenic bacteria is phosphorus limitation, an essential element for cellular processes. Here, we describe cellular and extracellular proteins differentially regulated between phosphate-deplete (0 mM, no added phosphate) and phosphate-replete (1 mM) growth conditions using a comparative proteomics (LC-MS/MS) approach. We observed a total of 128 and 65 unique proteins were downregulated and upregulated respectively, in the B. cenocepacia proteome. Of those downregulated proteins, many have functions in amino acid transport/metabolism. We have identified 24 upregulated proteins that are directly/indirectly involved in inorganic phosphate or organic phosphorus acquisition. Also, proteins involved in virulence and antimicrobial resistance were differentially regulated, suggesting B. cenocepacia experiences a dramatic shift in metabolism under these stress conditions. Overall, this study provides a baseline for further research into the biology of Burkholderia in response to phosphorus stress.
洋葱伯克霍尔德氏菌复合群是一组洋葱伯克霍尔德氏菌物种，它们是机会性病原体，导致囊性纤维化患者的死亡率很高。这些土壤栖息和致病性细菌经常遇到的环境压力是磷限制，这是细胞过程所必需的元素。在这里，我们使用比较蛋白质组学（LC-MS/MS）方法描述了在缺磷（0 mM，不添加磷酸盐）和磷充足（1 mM）生长条件下差异调节的细胞内和细胞外蛋白质。我们观察到在 B. cenocepacia 蛋白质组中分别下调和上调的总共有 128 和 65 个独特蛋白质。在下调的蛋白质中，许多具有氨基酸运输/代谢功能。我们已经鉴定出 24 个上调的蛋白质，它们直接/间接参与无机磷酸盐或有机磷的获取。此外，参与毒力和抗微生物耐药性的蛋白质也存在差异调节，表明在这些胁迫条件下，B. cenocepacia 的代谢发生了剧烈变化。总的来说，这项研究为进一步研究洋葱伯克霍尔德氏菌对磷胁迫的生物学提供了基础。
