Boehm K D, Hood R L, Ilan J
Department of Developmental Genetics and Anatomy, Case Western Reserve University, Cleveland, OH 44106.
Proc Natl Acad Sci U S A. 1988 May;85(10):3450-4. doi: 10.1073/pnas.85.10.3450.
A primary monolayer culture system from cockerel hepatocytes was established. The cultures synthesize and secrete proteins that comigrate with authentic serum proteins on polyacrylamide gels and are found in the same relative abundance. Addition of estradiol increased the synthesis of apoprotein B, found in very low density lipoprotein, under all culture conditions. Vitellogenin synthesis could not be induced directly by estradiol. However, when serum was obtained from cockerels injected with estradiol 4 days before blood collection and included in the culture medium, the cultures secreted a protein identified immunologically as vitellogenin by affinity chromatography. Furthermore, addition of growth hormone or prolactin to cultured cockerel hepatocyte monolayers resulted in the synthesis and secretion of a polypeptide that comigrates with authentic vitellogenin on polyacrylamide gels.
建立了来自公鸡肝细胞的原代单层培养系统。这些培养物合成并分泌在聚丙烯酰胺凝胶上与天然血清蛋白迁移率相同且相对丰度相同的蛋白质。在所有培养条件下，添加雌二醇都会增加极低密度脂蛋白中载脂蛋白B的合成。卵黄蛋白原的合成不能直接由雌二醇诱导。然而，当从采血前4天注射雌二醇的公鸡获得血清并将其包含在培养基中时，培养物分泌出一种通过亲和层析被免疫鉴定为卵黄蛋白原的蛋白质。此外，向培养的公鸡肝细胞单层中添加生长激素或催乳素会导致合成并分泌一种在聚丙烯酰胺凝胶上与天然卵黄蛋白原迁移率相同的多肽。
