Department of Applied Biology, School of Natural Science, Adama Science and Technology University, Adama, Ethiopia.
Department of Applied Biology, School of Natural Science, Adama Science and Technology University, Adama, Ethiopia.
Poult Sci. 2021 Apr;100(4):101004. doi: 10.1016/j.psj.2021.01.027. Epub 2021 Jan 19.
Newcastle disease (ND) is a major infectious disease of poultry caused by a virulent strain of Avian Paramyxovirus type-1 (APMV-1). It is a major threat to the poultry industry in many countries of the world including Ethiopia. The aim of this study was to conduct molecular surveillance of ND Virus and identify potential risk factors for nonvaccinated village chicken in Central Rift Valley of Oromia, Ethiopia. A total of 84 pooled swab samples, each made from pools of 5 swabs for analysis, from cloacal and tracheal sites of chickens in the Central Rift Valley were collected, and RNA was extracted to carry out real-time quantitative polymerase chain reaction. Out of the 84 pooled swab samples tested for M-gene, 13 (15.48%) samples were found positive for APMV-1. The prevalence of ND in males was found to be 16.10% and that in females was 14.67%. Although the overall ND prevalence was 15.48% (13/84), the highest prevalence was recorded in Adama, 42.86% (6/14), and no positive case was observed in Bote and Bishoftu (P < 0.05), while intermediate prevalence was obtained from Batu, Arsi-negele, and Shashemene (P > 0.05). In general, the present study provides important information on the epidemiology of ND based on M-gene assay in Central Rift Valley of Oromia, Ethiopia, and highlights the importance of implementing molecular surveillances practice in live poultry markets and village chickens.
新城疫（ND）是一种由禽副黏病毒 1 型（APMV-1）强毒株引起的家禽重大传染病。它是世界上许多国家，包括埃塞俄比亚的家禽业的主要威胁。本研究旨在对埃塞俄比亚奥罗米亚中部裂谷的非接种乡村鸡进行新城疫病毒的分子监测，并确定潜在的风险因素。共采集了 84 份来自中央裂谷的鸡的直肠和气管部位的混合拭子样本，每份样本由 5 个拭子混合而成进行分析，并提取 RNA 进行实时定量聚合酶链反应。在 84 份用于 M 基因检测的混合拭子样本中，有 13 份（15.48%）样本对 APMV-1 呈阳性。在雄性鸡中的 ND 患病率为 16.10%，在雌性鸡中的患病率为 14.67%。尽管 ND 的总患病率为 15.48%（13/84），但在阿达玛的患病率最高，为 42.86%（6/14），而在博泰与比绍夫图则未观察到阳性病例（P<0.05），而在巴图、阿尔西内盖莱和沙舍梅内则获得了中等的患病率（P>0.05）。总的来说，本研究基于 M 基因检测在埃塞俄比亚奥罗米亚中部裂谷提供了关于 ND 流行病学的重要信息，并强调了在活禽市场和乡村鸡中实施分子监测实践的重要性。
