Marin Clara, Marco-Jiménez Francisco, Martínez-Priego Llucia, De Marco-Romero Griselda, Soriano-Chirona Vicente, Lorenzo-Rebenaque Laura, D'Auria Giuseppe
Institute of Biomedical Sciences, Veterinary Faculty, Universidad Cardenal Herrera-CEU, 46113 Alfara del Patriarca, Spain.
Institute of Science and Animal Technology, Universitat Politècnica de València, 46022 Valencia, Spain.
Animals (Basel). 2022 Aug 13;12(16):2065. doi: 10.3390/ani12162065.
Campylobacter is recognised as one of the most important foodborne bacteria, with a worldwide health and socioeconomic impact. This bacterium is one of the most important zoonotic players in poultry, where efficient and fast detection methods are required. Current official culture methods for Campylobacter enumeration in poultry usually include >44 h of culture and >72 h for identification, thus requiring at least five working shifts (ISO/TS 10272-2:2017). Here, we have assembled a portable sequencing kit composed of the Bento Lab and the MinION and developed a workflow for on-site farm use that is able to detect and report the presence of Campylobacter from caecal samples in less than five hours from sampling time, as well as the relationship of Campylobacter with other caecal microbes. Beyond that, our workflow may offer a cost-effective and practical method of microbiologically monitoring poultry at the farm. These results would demonstrate the possibility of carrying out rapid on-site screening to monitor the health status of the poultry farm/flock during the production chain.
弯曲杆菌被认为是最重要的食源性病原体之一，对全球健康和社会经济都有影响。这种细菌是家禽中最重要的人畜共患病原体之一，因此需要高效快速的检测方法。目前用于家禽中弯曲杆菌计数的官方培养方法通常需要超过44小时的培养时间和超过72小时的鉴定时间，因此至少需要五个工作日轮班（ISO/TS 10272-2:2017）。在此，我们组装了一套由Bento Lab和MinION组成的便携式测序试剂盒，并开发了一种适用于农场现场使用的工作流程，该流程能够在采样后不到五小时内检测并报告盲肠样本中弯曲杆菌的存在情况，以及弯曲杆菌与其他盲肠微生物的关系。除此之外，我们的工作流程可能为农场微生物学监测家禽提供一种经济高效且实用的方法。这些结果将证明在生产链中对家禽养殖场/鸡群的健康状况进行快速现场筛查的可能性。
