Molecular Characterization of Hydatid Cysts Cases in a Wild Boar and Mule in Turkey.

OBJECTIVE

Cystic echinococcosis (CE) is a zoonotic infection that affects humans, livestock and wild animals through the larval form of (s.l.). Molecular and taxonomic studies carried out in the recent years accept that Echinococcus granulosus s.l., a complex of 5 cryptic species, causes CE. In this study, we performed morphological and molecular characterisation of cyst isolates obtained from a wild boar and mule naturally infected with hydatid cyst.

METHODS

After gDNA isolation, the mitochondrial cytochrome oxidase subunit 1 (mt-) gene region was amplified by polymerase chain reaction (PCR) with specific primers. The amplified mt- PCR products were purified and one-way DNA sequence analysis was performed.

RESULTS

Comparison of the partial sequences of mt gene from the hydatid cyst isolates with that of reference sequences in GenBank revealed 100% similarity with (G1-G3) sequences.

CONCLUSION

To the best of our knowledge, this is the first study to determine the molecular characterisation of Echinococcus species in a wild boar in Turkey.