Zait Houria, Kouidri Mokhtaria, Grenouillet Florence Elisabeth, Umhang Gérald, Millon Laurence, Hamrioui Boussad, Grenouillet Frédéric
Parasitology and Mycology department, Mustapha University Hospital, 16000, Algiers, Algeria.
Institute of Veterinary Science, Ibn-khaldoun University of Tiaret, 14000, Tiaret, Algeria.
Parasitol Res. 2016 Jun;115(6):2423-31. doi: 10.1007/s00436-016-4994-5. Epub 2016 Mar 28.
In Algeria, previous studies investigated genotypes of Echinococcus granulosus sensu lato in animals and identified E. granulosus sensu stricto (s.s.) genotypes G1 and G3 whereas Echinococcus canadensis genotype G6 was only reported from dromedary cysts. Molecular data on human cystic echinococcosis (CE) were limited. We implemented a large genotyping study of hydatid cysts from humans and livestock animals to specify CE's molecular epidemiology and the genetic diversity in Algeria. Fifty-four human CE cysts from patients predominantly admitted in surgical units from Mustapha Hospital, Algiers, and 16 cysts from livestock animals gathered in two geographically distinct slaughterhouses, Tiaret and Tamanrasset, were collected. Molecular characterization was performed using sequencing of two mitochondrial genes, cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit I (NDI). In humans, G1 of E. granulosus s.s. was the main genotype (90.7 %); four samples (7.4 %) were characterized as E. granulosus s.s. G3 and one cyst as E. canadensis G6 (1.8 %). This molecular confirmation of E. canadensis G6 human infection in Algeria was observed in a Tuareg female living in a desertic area in Tamanrasset. All cysts from sheep, cattle, and goat were identified as E. granulosus s.s. G1 and the two cysts originating from dromedary as E. canadensis G6. Twenty concatenated haplotypes (COI + NDI) were characterized. Among E. granulosus s.s., one haplotype (HL1) was highly predominant in both humans and animals cysts (71.6 %). This study revealed main occurrence of E. granulosus s.s. in humans and livestock animals, with description of a predominant shared haplotype corresponding to the main worldwide observed haplotype E.granulosus s.s. G1. E. canadensis G6 was limited to South Algeria, in dromedary as well as in human.
在阿尔及利亚，以往的研究调查了动物体内细粒棘球绦虫复合种的基因型，并鉴定出狭义细粒棘球绦虫（E. granulosus sensu stricto，s.s.）的G1和G3基因型，而加拿大棘球绦虫G6基因型仅在单峰驼囊肿中被报道过。关于人类囊型包虫病（CE）的分子数据有限。我们开展了一项针对人类和家畜包虫囊肿的大型基因分型研究，以明确阿尔及利亚CE的分子流行病学及遗传多样性。我们收集了54例主要来自阿尔及尔穆斯塔法医院外科病房患者的人类CE囊肿，以及在两个地理位置不同区域的屠宰场（提亚雷特和塔曼拉塞特）采集的16例家畜囊肿。利用细胞色素c氧化酶亚基I（COI）和NADH脱氢酶亚基I（NDI）这两个线粒体基因进行测序以进行分子特征分析。在人类中，狭义细粒棘球绦虫G1是主要基因型（90.7%）；4个样本（7.4%）被鉴定为狭义细粒棘球绦虫G3，1个囊肿为加拿大棘球绦虫G6（1.8%）。在阿尔及利亚，对一名居住在塔曼拉塞特沙漠地区的图阿雷格族女性进行的检测中发现了加拿大棘球绦虫G6感染人类的分子证据。所有来自绵羊、牛和山羊的囊肿均被鉴定为狭义细粒棘球绦虫G1，而来自单峰驼的两个囊肿为加拿大棘球绦虫G6。共鉴定出20种串联单倍型（COI + NDI）。在狭义细粒棘球绦虫中，一种单倍型（HL1）在人类和动物囊肿中均占主导地位（71.6%）。本研究揭示了狭义细粒棘球绦虫在人类和家畜中占主要比例，并描述了一种占主导地位的共享单倍型，该单倍型与全球主要观察到的狭义细粒棘球绦虫G1单倍型相对应。加拿大棘球绦虫G6仅限于阿尔及利亚南部，在单峰驼和人类中均有发现。
