Department of Gastroenterology, The People's Hospital of China Three Gorges University and The First People's Hospital of Yichang, Yichang, Hubei Province, China.
Bioengineered. 2021 Dec;12(1):886-897. doi: 10.1080/21655979.2021.1889130.
Circular RNAs (CircRNAs), belonging to non-coding RNAs, exert a crucial modulatory role in cancer progression. In this study, circRNA microarray analysis was utilized to screen differentially expressed circRNA in colorectal cancer (CRC) and circ_0000467 was identified as one circRNA whose expression was significantly upregulated in CRC. Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) indicated that circ_0000467 and engrailed-2 (EN2) expression levels were up-modulated, while the expression level of miR-382-5p was down-modulated in CRC tissues. The depletion of circ_0000467 expression was found to impede the multiplication, migration, invasion, and epithelial-mesenchymal transition (EMT) processes in CRC cells, which were examined by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and Transwell experiments. Dual-luciferase reporter assay was used to verify the targeting relationship between circ_0000467 and miR-382-5p. It was also revealed that circ_0000467 could up-regulate EN2 expression via repressing miR-382-5p in CRC cells. Furthermore, EN2 overexpression counteracted the suppressing effects of circ_0000467 knockdown on the malignant behaviors of CRC cells. To sum up, circ_0000467 facilitates CRC development by modulating the miR-382-5p/EN2 axis, and circ_0000467 is a promising target for CRC therapy.
环状 RNA（circRNAs）属于非编码 RNA，在癌症进展中发挥着关键的调节作用。在这项研究中，利用环状 RNA 微阵列分析筛选结直肠癌（CRC）中差异表达的环状 RNA，鉴定出circ_0000467 是一种在 CRC 中表达显著上调的环状 RNA。实时定量逆转录-聚合酶链反应（qRT-PCR）显示，circ_0000467 和 engrailed-2（EN2）的表达水平上调，而 miR-382-5p 的表达水平下调在 CRC 组织中。circ_0000467 表达的耗竭被发现会阻碍 CRC 细胞的增殖、迁移、侵袭和上皮-间充质转化（EMT）过程，这通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐（MTT）和 Transwell 实验进行了检查。双荧光素酶报告基因实验用于验证 circ_0000467 和 miR-382-5p 之间的靶向关系。还表明，circ_0000467 可以通过抑制 CRC 细胞中的 miR-382-5p 而上调 EN2 表达。此外，EN2 的过表达抵消了 circ_0000467 敲低对 CRC 细胞恶性行为的抑制作用。总之，circ_0000467 通过调节 miR-382-5p/EN2 轴促进 CRC 的发展，circ_0000467 是 CRC 治疗的有前途的靶点。
